Two levels of regulation of beta-interferon gene expression in human cells.
Two levels of regulation of beta-interferon gene expression in human cells.
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人类细胞中β-干扰素基因表达的两个水平的调节。
DOI：
10.1073/pnas.80.13.3923
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发表时间：
1983
影响因子：
11.1
通讯作者：
Pitha,PM
中科院分区：
文献类型：
--
作者：
Raj,NB;Pitha,PM
我们克隆了α-和β-干扰素cDNA，并将它们用作特异性探针，以测定聚（rI）、聚（rC）或纽卡斯尔病病毒诱导人成纤维细胞合成干扰素时干扰素mRNA的相对水平。两种诱导剂都只激活β-干扰素基因;然而，病毒诱导的细胞中β-干扰素mRNA的半衰期明显长于poly（rI）、poly（rC）诱导的细胞。在从poly（rI）、poly（rC）诱导的细胞中分离的细胞核中检查β-干扰素RNA序列的转录速率;发现诱导导致β-干扰素基因的转录激活，并且当没有检测到干扰素合成或细胞质β-干扰素RNA时为关闭期。因此，在poly（rI）、poly-（rC）诱导的人成纤维细胞中β-干扰素的合成受到β-干扰素基因转录的激活和β-干扰素mRNA稳定性的改变的控制。
We cloned alpha- and beta-interferon cDNA and used them as specific probes to determine the relative levels of interferon mRNA in human fibroblasts cells induced with poly(rI).poly(rC) or Newcastle disease virus to synthesize interferon. Both inducers activated only the beta-interferon gene; however, the half life of beta-interferon mRNA in cells induced with virus was substantially longer than in poly(rI).poly(rC)-induced cells. The transcription rate of beta-interferon RNA sequences was examined in nuclei isolated from poly(rI).poly(rC)-induced cells; it was found that the induction leads to transcriptional activation of the beta-interferon gene and that the shutoff period when no interferon synthesis or cytoplasmic betamRNA are detected. Thus, the synthesis of beta interferon in poly(rI).poly-(rC)-induced human fibroblasts is controlled both by activation of transcription of the beta-interferon gene and by alteration of the beta-interferon mRNA stability.