Evolutionary conservation analysis between the essential and nonessential genes in bacterial genomes.
Evolutionary conservation analysis between the essential and nonessential genes in bacterial genomes.
复制标题
细菌基因组必需基因和非必需基因之间的进化保守性分析
DOI：
10.1038/srep13210
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发表时间：
2015-08-14
影响因子：
4.6
通讯作者：
Lin Y
中科院分区：
文献类型：
--
作者：
Luo H;Gao F;Lin Y
必需基因被认为是生物体在一定条件下生存的关键，作用于必需基因的自然选择比作用于非必需基因的自然选择更为严格。到目前为止，已经通过实验方法在大约30种细菌生物中鉴定了必需基因。在本文中，我们进行了全面的比较之间的必需和非必需基因在23种细菌的基因组的基础上Ka/Ks比值，发现必需基因比非必需基因在进化上更保守，在大多数的细菌检查。此外，我们还分析了功能簇的保守性，发现G.（碳水化合物运输和代谢），H（辅酶转运和代谢），我（转录），J（翻译，核糖体结构和生物发生），K（脂质转运和代谢）和L（复制，重组和修复）往往比细菌中相应的非必需基因更保守。这些结果表明，这些亚类中的必需基因比非必需基因受到更强的选择压力，从而为复杂生物过程中必需基因和非必需基因的进化保守性提供了更多的见解。
Essential genes are thought to be critical for the survival of the organisms under certain circumstances and the natural selection acting on essential genes is expected to be stricter than on nonessential ones. Up to now, essential genes have been identified in approximately thirty bacterial organisms by experimental methods. In this paper, we performed a comprehensive comparison between the essential and nonessential genes in the genomes of 23 bacterial species based on the Ka/Ks ratio and found that essential genes are more evolutionarily conserved than nonessential genes in most of the bacteria examined. Furthermore, we also analyzed the conservation by functional clusters with the clusters of orthologous groups (COGs) and found that the essential genes in the functional categories of G (Carbohydrate transport and metabolism), H (Coenzyme transport and metabolism), I (Transcription), J (Translation, ribosomal structure and biogenesis), K (Lipid transport and metabolism) and L (Replication, recombination and repair) tend to be more evolutionarily conserved than the corresponding nonessential genes in bacteria. The results suggest that the essential genes in these subcategories are subject to stronger selective pressure than the nonessential genes and therefore, provide more insights of the evolutionary conservation for the essential and nonessential genes in complex biological processes.