Antibacterial activity and mechanism of thymol against Alicyclobacillus acidoterrestris vegetative cells and spores
Antibacterial activity and mechanism of thymol against Alicyclobacillus acidoterrestris vegetative cells and spores
复制标题
百里香酚对酸土脂环酸芽孢杆菌营养细胞和孢子的抗菌活性及机制
DOI：
10.1016/j.lwt.2019.01.066
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发表时间：
2019-05-01
影响因子：
6
通讯作者：
Yue, Tianli
中科院分区：
文献类型：
--
作者：
Cai, Rui;Zhang, Mingquan;Yue, Tianli
耐酸脂肪杆菌因其独特的耐热性和在巴氏杀菌果汁变质中的应用而备受关注。本研究旨在探讨百里香酚对嗜酸杆菌营养细胞和孢子的抑菌特性及其机制。百里香酚对营养细胞的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)均为0.25 mg/mL。百里香酚对孢子的最低抑菌浓度为0.5 mg/m L，最低杀菌浓度大于1.0 mg/m L。百里香酚能有效地抑制A.acidoterrestris的生长、孢子萌发和愈创木酚的产生。百里香酚处理后，酸性木霉细胞和孢子的核酸和蛋白质渗漏显著增加。菌体蛋白质的SDS-PAGE分析表明，可溶性蛋白质的损失也很明显。扫描电子显微镜观察表明，百里香酚处理后的酸性麦冬细胞或孢子有严重的形态破坏。这些结果表明，百里香酚通过破坏细胞和孢子的完整性而发挥了良好的抗菌活性。本研究为果汁中嗜酸杆菌的灭活提供了一种新的方法。
Alicyclobacillus acidoterrestris has received much attention due to its unique thermo-acidophilic property and implication in the spoilage of pasteurized juices. The aim of this study was to investigate the antibacterial characteristics and mechanisms of thymol against A. acidoterrestris vegetative cells and spores. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of thymol against vegetative cells were both 0.25 mg/mL. MIC of thymol against spores was 0.5 mg/mL, and the MBC was greater than 1.0 mg/mL. Thymol could effectively inhibit A. acidoterrestris growth, spore germination and guaiacol production. When treated by thymol, the leakage of nucleic acids and proteins from A. acidoterrestris cells and spores increased significantly. SDS-PAGE investigation of bacterial proteins showed that the loss of soluble proteins was obvious as well. Scanning electron microscopy observation exhibited that A. acidoterrestris cells or spores treated with thymol had a severe morphological damage. These results demonstrated that thymol exerted its good antibacterial activities by destroying the integrity of the cell and spore. This study provides a new method for the inactivation of A. acidoterrestris in fruit juice.