Co-occurrence of antibiotic and metal resistance genes revealed in complete genome collection
Co-occurrence of antibiotic and metal resistance genes revealed in complete genome collection
复制标题
DOI：
10.1038/ismej.2016.155
复制
发表时间：
2017-03-01
期刊：
影响因子：
11
通讯作者：
Zhang, Tong
中科院分区：
文献类型：
--
作者：
Li, Li-Guan;Xia, Yu;Zhang, Tong
抗生素耐药的高频率是一个全球公共卫生问题。更严重的是，环境中广泛存在的金属压力可能通过抗生素耐药基因（ARGs）和金属耐药基因（MRGs）的共选择促进抗生素耐药性的增殖。鉴于缺乏对ARG和MRG共选择的全面了解，本研究通过对大量完整基因组收集进行基因组分析，严格研究了ARG和MRG之间的丰度关系和遗传连锁。与那些受到较少人为干扰的ARGs相比，更多的ARGs在人类相关细菌中富集。在人类病原体中，ARG和MRG共同出现的特征要比那些与人类关联较少的细菌频繁得多，ARG和MRG之间的距离联系要密切得多。此外，不同生境分区的共现结构也存在显著差异，这可能与不同生境分区的基因转移潜力有关。人类病原菌基因组上更多的外源ARGs和MRGs表明，近期耐药性获得在人类共生菌群的抗性组发育中具有重要意义。总的来说，该研究强调了ARG和MRG共同选择的环境和医学相关性的潜在风险。
The high frequency of antibiotic resistance is a global public health concern. More seriously, widespread metal pressure in the environment may facilitate the proliferation of antibiotic resistance via coselection of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs). Given the lack of comprehensive understanding of the ARG and MRG coselection, in this study both abundance relationship and genetic linkage between ARGs and MRGs were rigorously investigated by performing a genomic analysis of a large complete genome collection. Many more ARGs were enriched in human-associated bacteria compared with those subjected to less anthropogenic interference. The signatures of ARG and MRG co-occurrence were much more frequent and the distance linkages between ARGs and MRGs were much more intimate in human pathogens than those less human-associated bacteria. Moreover, the co-occurrence structures in the habitat divisions were significantly different, which could be attributed to their distinct gene transfer potentials. More exogenous ARGs and MRGs on the genomes of human pathogens indicated the importance of recent resistance acquisition in resistome development of human commensal flora. Overall, the study emphasizes the potential risk associated with ARG and MRG coselection of both environmental and medical relevance.