Vascular endothelial growth factor increases hydraulic conductivity of isolated perfused microvessels
Vascular endothelial growth factor increases hydraulic conductivity of isolated perfused microvessels
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DOI：
10.1152/ajpheart.1996.271.6.h2520
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发表时间：
1996-12-01
影响因子：
4.8
通讯作者：
Curry, FE
中科院分区：
文献类型：
--
作者：
Bates, DO;Curry, FE
这些实验报告了在暴露于血管内皮生长因子(VEGF)后首次直接测量微血管通透性系数。将Landis技术扩展到能够测量青蛙肠系膜微血管对水流的阻力，即水力传导性(L(P))，在最初暴露于血管内皮生长因子(急性)以及首次暴露后24小时和72小时(慢性)期间，测量同一微血管的水力传导性。L(P)的对照测量值无论是急性还是慢性都没有变化。暴露于1 nM的血管内皮生长因子后，微血管L(P)在30分钟内迅速增加(达到基线的7.8倍)，并在2分钟内恢复到对照组。血管内皮细胞血管内皮细胞生长因子灌流24 h后，血管内皮细胞L(P)值较治疗前增加5倍，72 h后恢复至原值。这些实验证实了血管内皮细胞生长因子具有急性(几分钟)和慢性(几小时)双重作用的假说。
These experiments report the first direct measurement of microvessel permeability coefficients after exposure to vascular endothelial growth factor (VEGF). The Landis technique was extended to enable measurement of the resistance of the microvessel wall to water flow, hydraulic conductivity (L(p)), on the same microvessel in the frog mesentery during the initial exposure to VEGF (acute) and 24 and 72 h after initial exposure (chronic). Control measurements of L(p) showed no change either acutely or chronically. Exposure to 1 nM VEGF rapidly and transiently increased microvessel L(p) within 30 s (to 7.8-fold greater than baseline values) and returned to control within 2 min. The baseline L(p) was fivefold greater after 24 h than the initial baseline as a result of VEGF perfusion and returned to its original value after 72 h. These experiments confirm the hypothesis that VEGF acts both acutely (over a period of a few minutes) and chronically (over a few hours) to increase microvascular permeability.