Enhancing Synovial Mesenchymal Stem Cell Adhesion and Selection via an Avidin-Biotin-CD105 Binding System for Cartilage Tissue Engineering
Enhancing Synovial Mesenchymal Stem Cell Adhesion and Selection via an Avidin-Biotin-CD105 Binding System for Cartilage Tissue Engineering
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DOI：
10.1166/jbt.2016.1401
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发表时间：
2016-01-01
影响因子：
0.1
通讯作者：
Guo, Changan
中科院分区：
文献类型：
--
作者：
Fan, Wenshuai;Wang, Yiming;Guo, Changan
在许多软骨组织工程策略中，细胞与支撑支架的有效附着是关键步骤。本研究旨在探讨新型抗生物素蛋白-生物素偶联的CD 105抗体结合系统（CD 105-ABBS）对滑膜间充质干细胞（SMSC）在软骨组织工程中粘附和选择的影响。通过计数粘附细胞来评估SMSC在细胞培养板或多孔支架上的粘附，并通过免疫荧光和1，9-二甲基亚甲基蓝结合试验来评估细胞培养板上的CD 105阳性细胞以及多孔支架中的硫酸化糖胺聚糖（sGAG）分泌。最后，采用不同的结合系统，构建了含细胞的支架材料，修复了比格犬股骨髁部的缺损，并检测了其sGAG和胶原II的含量。结果表明，CD 105-ABBS改善了SMSC粘附，尤其是在接种后的早期时间点。此外，CD 105-ABBS促进CD 105阳性细胞的选择以及sGAG的分泌。此外，在体内使用CD 105-ABBS改善了植入的SMSCs的修复效果。因此，本研究的结果表明，这种新的结合系统不仅可以增强SMSC粘附，而且还可以改善对特定SMSC子集的选择，以支持软骨修复。
Efficient attachment of cells to a supporting scaffold is the critical step in many cartilage tissue engineering strategies. The present study aimed to investigate the effects of a novel avidin-biotin-conjugated CD105 antibody binding system (CD105-ABBS) on synovial mesenchymal stem cell (SMSC) adhesion and selection in cartilage tissue engineering. SMSC adhesion on cell culture plates or porous scaffolds was assessed by counting attached cells, and CD105-positive cells on cell culture plates as well as sulfated glycosaminoglycan (sGAG) secretion in porous scaffolds were evaluated by immunofluorescence and the 1, 9-dimethylmethylene blue binding assay. Finally, defects created in the femoral condyle of beagles were repaired using cell-laden scaffolds employing different binding system, and the contents of sGAG and collagen II in the engineered cartilage were evaluated. The results indicated that the CD105-ABBS improved SMSC adhesion, especially at early time points after seeding. In addition, the CD105-ABBS promoted the selection of CD105-positive cells as well as the secretion of sGAG. Moreover, the reparative effects of implanted SMSCs were improved with the use of the CD105-ABBS in vivo. Therefore, the results of this study suggest that this new binding system can not only enhance SMSC adhesion, but also improve selection of a specific subset of SMSCs, in support of cartilage repair.