Tetrahymena Poc1 ensures proper intertriplet microtubule linkages to maintain basal body integrity
Tetrahymena Poc1 ensures proper intertriplet microtubule linkages to maintain basal body integrity
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DOI：
10.1091/mbc.e16-03-0165
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发表时间：
2016-08-01
影响因子：
3.3
通讯作者：
Winey, Mark
中科院分区：
文献类型：
--
作者：
Meehl, Janet B.;Bayless, Brian A.;Winey, Mark
基底体由九个对称的三重微管组成，它们锚定由活动纤毛的不对称搏动模式产生的力。纤毛蛋白Poc 1通过一种未知的机制稳定基体。在poc 1 Delta细胞中，电子断层扫描揭示了连接相邻三重微管的三重态间连接体（A-C连接体）组织中的细微缺陷。完整的三联体微管丢失优先在基体的后面附近。缺失三联体的基体可能仍然有能力组装具有9个三联体微管的新基体，这表明母体基体微管结构不模板化子体。我们的数据表明，Poc 1稳定基体三联微管通过相邻的三联体之间的连接。如果没有这种稳定性，在基体内的特定的三联体微管更容易损失，可能是由于在纤毛跳动期间基体内的力分布。这项工作提供了深入了解纤毛蛋白Poc 1如何保持基体的完整性。
Basal bodies comprise nine symmetric triplet microtubules that anchor forces produced by the asymmetric beat pattern of motile cilia. The ciliopathy protein Poc1 stabilizes basal bodies through an unknown mechanism. In poc1 Delta cells, electron tomography reveals subtle defects in the organization of intertriplet linkers (A-C linkers) that connect adjacent triplet microtubules. Complete triplet microtubules are lost preferentially near the posterior face of the basal body. Basal bodies that are missing triplets likely remain competent to assemble new basal bodies with nine triplet microtubules, suggesting that the mother basal body microtubule structure does not template the daughter. Our data indicate that Poc1 stabilizes basal body triplet microtubules through linkers between neighboring triplets. Without this stabilization, specific triplet microtubules within the basal body are more susceptible to loss, probably due to force distribution within the basal body during ciliary beating. This work provides insights into how the ciliopathy protein Poc1 maintains basal body integrity.