QUANTITATION AND IGG SUBCLASS DISTRIBUTION OF ANTICHROMATIN AUTOANTIBODIES IN SLE MICE
QUANTITATION AND IGG SUBCLASS DISTRIBUTION OF ANTICHROMATIN AUTOANTIBODIES IN SLE MICE
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DOI：
10.1016/0090-1229(88)90183-3
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发表时间：
1988-02-01
期刊：
影响因子：
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通讯作者：
COHEN, PL
中科院分区：
文献类型：
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作者：
FISHER, CL;EISENBERG, RA;COHEN, PL
在大多数患有自发性系统性红斑狼疮的小鼠中发现了天然染色质制剂的抗体。所有检测的MRL/MP-LPR/LPR(MRL/LPR)血清(>500份)均含有染色质抗体，且抗染色质水平随年龄增长而升高。在MRL/LPR血清中，大约50%的抗染色质抗体属于IgG2a亚类，30%为IgG2b，10%为IgG1，10%为IgG3。有趣的是，抗染色质自身抗体对IgG2a亚类的相对限制在MRL/LPR小鼠中很明显，早在淋巴结病发生之前的1个月。在MRL/MP-+/+(MRL/+)、NZB、(NZB)血清中也检测到抗染色质自身抗体。NZW)F1(B.Times.W)，和BXSB小鼠，但在正常小鼠血清中未检测到。MRL/+、B.Times的亚类分布类似，偏向IgG2a。W、NZB小鼠。这些结果表明，针对染色质的自发自身抗体是小鼠系统性红斑狼疮的良好标志物，并且主要属于IgG2a亚类。
Antibodies to a native chromatin preparation were found in most mice suffering from spontaneous SLE. All MRL/Mp-lpr/lpr (MRL/lpr) sera tested (more than 500) contained antibodies to chromatin and antichromatin levels increased with age. Approximately 50% of the IgG antichromatin antibody in the MRL/lpr sera was of the IgG2a subclass, 30% IgG2b, 10% IgG1, and 10% IgG3. Interestingly, the relative restriction of antichromatin autoantibodies to the IgG2a subclass was apparent in MRL/lpr mice as young as 1 month, well before the onset of lymphadenopathy. Antichromatin autoantibodies were also detectable in sera from MRL/Mp- +/+ (MRL/+), NZB, (NZB .times. NZW)F1 (B .times. W), and BXSB mice, but were not found in sera from normal mice. A similar subclass distribution skewed toward IgG2a was seen for MRL/+, B .times. W, and NZB mice. These results indicate that the spontaneous autoantibody directed against chromatin is a good marker for murine SLE, and is predominantly of the IgG2a subclass.