SELECTIVE LOCALIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE AND SUBSTANCE-P IN HUMAN EOSINOPHILS
SELECTIVE LOCALIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE AND SUBSTANCE-P IN HUMAN EOSINOPHILS
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DOI：
10.1016/s0006-291x(87)80293-0
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发表时间：
1987-11-13
影响因子：
3.1
通讯作者：
GOETZL, EJ
中科院分区：
文献类型：
--
作者：
ALIAKBARI, J;SREEDHARAN, SP;GOETZL, EJ
纯化的人嗜酸性粒细胞提取物的免疫反应性血管活性肠肽的平均浓度为72 fmol和21 fmol的P物质每107个嗜酸性粒细胞，这是显着高于中性粒细胞，单核白细胞和血小板中相同的神经肽的免疫反应性的内容。相反，降钙素基因相关肽和生长抑素的浓度较低，在所有白细胞的提取物是相似的。嗜酸性粒细胞的肽的色谱法证实了它们的身份与血管活性肠肽和P物质的神经内分泌来源。某些神经肽的储存可能赋予嗜酸性粒细胞在宿主防御和超敏反应中的独特作用。
Extracts of purified human eosinophils had a mean concentration of 72 fmol of immunoreactive vasoactive intestinal peptide and 21 fmol of substance P per 107 eosinophils, that were significantly higher than the content of immunoreactivity of the same neuropeptides in neutrophils, mononuclear leukocytes, and platelets. In contrast, the lower concentrations of calcitonin gene-related peptide and somatostatin were similar in extracts of all leukocytes. Chromatography of the peptides from eosinophils confirmed their identity with vasoactive intestinal peptide and substance P from neuroendocrine sources. Stores of some neuropeptides may endow eosinophils with unique roles in host defense and hypersensitivity reactions.