Solubilized adenosine receptors in the brain: regulation of guanine nucleotides.
Solubilized adenosine receptors in the brain: regulation of guanine nucleotides.
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大脑中溶解的腺苷受体：鸟嘌呤核苷酸的调节。
DOI：
10.1126/science.6280275
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
Gavish,M;Goodman,RR;Snyder,SH
与腺苷酸环化酶的减少相关的腺苷受体，并由氚标记的环己基腺苷标记，可以用胆酸钠从脑细胞膜中溶解。鸟嘌呤核苷酸对受体结合的调节保持在可溶状态。在腺苷受体的膜制备物中观察到的阳离子的影响不再用溶解的受体检测到。受体和三磷酸鸟苷结合蛋白而不是阳离子结合蛋白的复合物在可溶状态下的表观保留可能允许对受体调节进行分子分析。
Adenosine receptors associated with a reduction of adenylate cyclase and labeled by tritium-labeled cyclohexyladenosine can be solubilized from brain membranes with sodium cholate. Regulation of receptor binding by guanine nucleotides is retained in the soluble state. Influences of cations observed in membrane preparations of adenosine receptors are no longer detected with the solubilized receptors. The apparent retention of a complex of receptors and guanosine triphosphate binding but not cation binding protein in the soluble state may permit a molecular analysis of receptor regulation.