Characterisation of nucleolar proteins as autoantigens using human autoimmune sera.
Characterisation of nucleolar proteins as autoantigens using human autoimmune sera.
复制标题
使用人自身免疫血清表征核仁蛋白作为自身抗原。
DOI：
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发表时间：
1986
影响因子：
27.4
通讯作者：
M. Franke
中科院分区：
文献类型：
--
作者：
J. Pfeifle;F. Anderer;M. Franke
应用免疫荧光法对271例全身性风湿病患者血清进行抗核仁抗体筛查。在73%的进行性系统性硬化症患者、4%的类风湿性关节炎自身抗体阳性患者和8%的类风湿性关节炎伴干燥综合征患者的血清中发现了抗核仁抗体，但在系统性红斑狼疮、干燥综合征、未分化结缔组织病或健康供体中未发现。抗核仁血清（n = 20）进行了分析，免疫印迹技术。在4种血清中均能检测到抗核仁蛋白的抗体，其分子量分别为35 kD、37 kD、69 kD、92-93 kD和93 kD。核仁自身抗原与前核糖体颗粒组分一起从核仁中提取，并可通过核糖核酸酶（RNAse）处理溶解。它们在细胞核中的存在对放线菌素D处理的细胞敏感。37 kD的自身抗原可被鉴定为核仁磷蛋白B23。
Sera from 271 patients with systemic rheumatic diseases were screened for antinucleolar antibodies using immunofluorescence. Antinucleolar antibodies were found in the sera of 73% of patients with progressive systemic sclerosis, 4% of patients positive for autoantibodies with rheumatoid arthritis, and 8% of patients with rheumatoid arthritis associated with sicca complex, but not in patients with systemic lupus erythematosus, Sjögren's syndrome, undifferentiated connective tissue disease, or in healthy donors. The antinucleolar sera (n = 20) were analysed by immunoblotting techniques. In four sera antibodies against nucleolar proteins with molecular weights of 35 kD, 37 kD, 69 kD, 92-93 kD, and 93 kD could be immunodetected. The nucleolar autoantigens were extractable from the nucleoli together with the preribosomal particle fraction and could be solubilised by ribonuclease (RNAse) treatment. Their presence in the nucleolus was sensitive to actinomycin D treatment of cells. The 37 kD autoantigen could be identified as nucleolar phosphoprotein B23.
核仁 7-2 RNA 与两种不同自身抗原的顺序关联。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hashimoto,C;Steitz,JA
通讯作者：
Steitz,JA