Genetic and antigenic characterisation of influenza A(H3N2) viruses isolated in Yokohama during the 2016/17 and 2017/18 influenza seasons
Genetic and antigenic characterisation of influenza A(H3N2) viruses isolated in Yokohama during the 2016/17 and 2017/18 influenza seasons
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DOI：
10.2807/1560-7917.es.2019.24.6.1800467
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发表时间：
2019-02-07
期刊：
影响因子：
19
通讯作者：
Kawaoka, Yoshihiro
中科院分区：
文献类型：
--
作者：
Kawakami, Chiharu;Yamayoshi, Seiya;Kawaoka, Yoshihiro
背景：甲型H3N2流感病毒快速进化以逃避人类免疫反应，导致血凝素（HA）抗原性发生变化。因此，有必要对甲型H3N2病毒进行连续的遗传和抗原分析，以尽快检测出抗原突变体。目的：我们试图从系统发育和抗原学角度捕捉2016/17和2017/18流感季节日本横滨甲型H3N2病毒感染的流行趋势。方法：我们测定了 2016/17 和 2017/18 流感季期间在日本横滨检测到的 A(H3N2) 病毒的 HA 序列，以确定 HA 中的氨基酸取代和潜在 N-糖基化位点的丢失或增加，这两者都可能影响 HA 的抗原性。我们还使用从实验感染的雪貂中获得的雪貂抗血清检查了分离株的抗原性。结果：2016/2017流感季节，检测到属于6个进化枝（进化枝3C.2A1、3C.2A1a、3C.2A1b、3C.2A2、3C.2A3和3C.2A4）的甲型（H3N2）流感病毒，同时检测到属于2个进化枝（进化枝3C.2A1b和3C.2A4）的甲型H3N2流感病毒。 2A2）在 2017/18 流感季节占主导地位。进化枝 3C 中的分离株。相对于其他进化枝，2A1a 和 3C.2A3 在 HA 中丢失了一个 N 连接糖基化位点。抗原分析揭示了进化枝之间的抗原差异，尤其是进化枝3C.2A2和3C.2A4病毒，它们在抗原图谱中显示出彼此之间以及与其他进化枝之间的明显抗原差异。结论：2016/17 和 2017/18 流感季节期间，多个分支在日本横滨共同流行，其中一些分支在抗原上与其他分支不同。
Background: Influenza A(H3N2) virus rapidly evolves to evade human immune responses, resulting in changes in the antigenicity of haemagglutinin (HA). Therefore, continuous genetic and antigenic analyses of A(H3N2) virus are necessary to detect antigenic mutants as quickly as possible. Aim: We attempted to phylogenetically and antigenically capture the epidemic trend of A(H3N2) virus infection in Yokohama, Japan during the 2016/17 and 2017/18 influenza seasons. Methods: We determined the HA sequences of A(H3N2) viruses detected in Yokohama, Japan during the 2016/17 and 2017/18 influenza seasons to identify amino acid substitutions and the loss or gain of potential N-glycosylation sites in HA, both of which potentially affect the antigenicity of HA. We also examined the antigenicity of isolates using ferret antisera obtained from experimentally infected ferrets. Results: Influenza A(H3N2) viruses belonging to six clades (clades 3C. 2A1, 3C. 2A1a, 3C. 2A1b, 3C. 2A2, 3C.2A3 and 3C.2A4) were detected during the 2016/17 influenza season, whereas viruses belonging to two clades (clades 3C. 2A1b and 3C. 2A2) dominated during the 2017/18 influenza season. The isolates in clades 3C. 2A1a and 3C.2A3 lost one N-linked glycosylation site in HA relative to other clades. Antigenic analysis revealed antigenic differences among clades, especially clade 3C.2A2 and 3C.2A4 viruses, which showed distinct antigenic differences from each other and from other clades in the antigenic map. Conclusion: Multiple clades, some of which differed antigenically from others, co-circulated in Yokohama, Japan during the 2016/17 and 2017/18 influenza seasons.