Immobilization of horseradish peroxidase on electrospun microfibrous membranes for biodegradation and adsorption of bisphenol A.
Immobilization of horseradish peroxidase on electrospun microfibrous membranes for biodegradation and adsorption of bisphenol A.
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DOI：
10.1016/j.biortech.2013.09.030
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发表时间：
2013-12
影响因子：
11.4
通讯作者：
Ran Xu;Chenglong Chi;Fengting Li;Bingru Zhang
中科院分区：
文献类型：
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作者：
Ran Xu;Chenglong Chi;Fengting Li;Bingru Zhang
成功地将辣根过氧化物酶（HRP）固定在新型酶载体聚甲基丙烯酸甲酯-丙烯酸乙酯（PMMA - CEA）微纤维膜上，用于水中双酚A的去除。采用静电纺丝法制备了直径为300 ~ 500 nm的聚甲基丙烯酸甲酯（PMMA） CEA纤维膜。HRP共价固定在聚乙烯亚胺和戊二醛活化的微纤维表面。HRP负荷达到285 mg/g，固定化后酶活性为游离HRP的70%。固定化后HRP的稳定性和可重复使用性都得到了很大的提高。经过6次重复运行后，固定的HRP保留了大约50%的初始活性。与游离HRP（61%）和PFM（42%）相比，固定化HRP在3 h内对双酚A （BPA）的去除率（93%）显著提高。固定化HPR在改性的PMMA - CEA载体上吸附，提高了HPR的催化活性，从而获得了较高的双酚a去除率。
Horseradish peroxidase (HRP) from roots of horseradish (Amoracia rusticana) was successfully immobilized on novel enzyme carriers, poly(methyl methacrylate-co-ethyl acrylate) (PMMA CEA) microfibrous membranes, and used for removal of bisphenol A from water. PMMA CEA fibrous membranes (PFM) with fiber diameters of 300–500 nm, were fabricated by electrospinning. HRP was covalently immobilized on the surface of microfibers previously activated by polyethylenimine and glutaraldehyde. HRP loading reached 285 mg/g, and enzyme activity was 70% of free HRP after immobilization. Both stabilities and reusability of HRP were greatly improved after immobilization. After six repeated runs, immobilized HRP retained about 50% of its initial activity. Immobilized HRP exhibited significantly higher removal efficiency for bisphenol A (BPA) in 3 h (93%) compared with free HRP (61%) and PFM alone (42%). The high BPA removal can be resulted by improvement of catalytic activity of immobilized HPR with adsorption on modified PMMA CEA support.