EXPRESSION OF THE W6/32 HLA EPITOPE BY CELLS OF RAT, MOUSE, HUMAN AND OTHER SPECIES - CRITICAL DEPENDENCE ON THE INTERACTION OF SPECIFIC MHC HEAVY-CHAINS WITH HUMAN OR BOVINE BETA-2-MICROGLOBULIN
EXPRESSION OF THE W6/32 HLA EPITOPE BY CELLS OF RAT, MOUSE, HUMAN AND OTHER SPECIES - CRITICAL DEPENDENCE ON THE INTERACTION OF SPECIFIC MHC HEAVY-CHAINS WITH HUMAN OR BOVINE BETA-2-MICROGLOBULIN
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DOI：
10.1002/eji.1830170907
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发表时间：
1987-09-01
影响因子：
5.4
通讯作者：
MACPHERSON, GG
中科院分区：
文献类型：
--
作者：
JEFFERIES, WA;MACPHERSON, GG
HLA I类表位W6/32在构象上依赖于重链和β。2-microglobulin (.beta.2M)。以前，W6/32表位已在人类和其他灵长类动物以及牛源中检测到。两个有争议的报告表明，W6/32表位在表达Db等位基因的正常或转化的小鼠细胞中都是组成性表达的。在这里，我们发现小鼠细胞中W6/32表位的出现是由于Db或Kd基因产物与牛或人的。β . 2m相关联。我们使用同源小鼠菌株和介于Db和Kb之间的杂交H-2类I基因，将W6/32表位定位到. α的特定氨基酸残基上。2域。随后，我们展示了。2M交换不局限于小鼠或人体外细胞，但可以在。β后检测到。小鼠注射2M。给出的数据表明。生理条件下，细胞表面发生2M交换，表明MHC I类重链在. β .2M的结合和非结合形式之间处于平衡状态。
The HLA class I epitope W6/32 is conformationally dependent on both heavy chain and .beta.2-microglobulin (.beta.2M). Previously, the W6/32 epitope has been detected in humans and other primates as well as from bovine sources. Two controversial reports suggest the W6/32 epitope is constitutively expressed by either normal or transformed murine cells expressing the Db allele. Here we show that the appearance of the W6/32 epitope in murine cells results from the association of either the Db or Kd gene products with either bovine or human .beta.2M. We use congenic mouse strains and hybrid H-2 class I genes between Db and Kb to map the W6/32 epitope to particular amino acid residues in the .alpha.2 domain. Subsequently, we show that .beta.2M exchange is not confined to murine or human cells in vitro but can be detected after .beta.2M injection into a mouse. The data presented suggests that .beta.2M exchange takes place at the cell surface under physiological conditions and indicates that MHC class I heavy chains are in an equilibrium between the bound and unbound form of .beta.2M.