Time-resolved Fluorescent Detection for Glucose Using a Complex of Luminescent Layered Titanates and Enzymes
Time-resolved Fluorescent Detection for Glucose Using a Complex of Luminescent Layered Titanates and Enzymes
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DOI：
10.2116/analsci.33.989
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发表时间：
2017-09
影响因子：
1.6
通讯作者：
Y. Sakaguchi;Tomoka Minamikawa;Mayuko Yamamuro;Tadayuki Tsujita;T. Ueda;K. Kamada;N. Soh
中科院分区：
文献类型：
--
作者：
Y. Sakaguchi;Tomoka Minamikawa;Mayuko Yamamuro;Tadayuki Tsujita;T. Ueda;K. Kamada;N. Soh
合成了铕掺杂的层状钛酸盐（Eu-TiOx）发光材料，并将其与辣根过氧化物酶（HRP）和葡萄糖氧化酶（GOx）配合。在愈创木酚的存在下加入葡萄糖后，所得Eu-TiOx/HRP/GOx复合物的发射降低。荧光强度随葡萄糖浓度的增加而降低，检测限为3.1 μM。该系统有望成为一种新的葡萄糖检测方法。
Luminescent europium-doped layered titanates (Eu-TiOx) were synthesized and complexed with horseradish peroxidase (HRP) and glucose oxidase (GOx). The emission of a resultant Eu-TiOx/HRP/GOx complex decreased upon the addition of glucose in the presence of guaiacol. The emission decrease was dependent on the concentrations of glucose, and the detection limit for glucose was 3.1 μM. The proposed system would be promising as a new detection method for glucose.