Antifungal activity of some Tanzanian plants used traditionally for the treatment of fungal infections
Antifungal activity of some Tanzanian plants used traditionally for the treatment of fungal infections
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DOI：
10.1016/j.jep.2006.04.026
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发表时间：
2006-11-03
影响因子：
5.4
通讯作者：
Verweij, Paul E.
中科院分区：
文献类型：
--
作者：
Hamza, Omar J. M.;den Beukel, Carolien J. P. van den Bout-van;Verweij, Paul E.
使用民族植物学的方法，一些坦桑尼亚的传统治疗者用于治疗口腔念珠菌病和皮肤真菌感染的植物报道，收集和筛选其抗真菌活性对白色念珠菌，光滑念珠菌，热带念珠菌，近平滑念珠菌，克柔念珠菌和新型隐球菌。根据临床和实验室标准研究所（CLSI）（前身为国家临床和实验室标准委员会）的指南[国家临床实验室标准委员会，2002年]，使用肉汤微量稀释法筛选了属于56种植物和38科的共65种粗甲醇提取物。酵母菌肉汤稀释抗真菌药敏试验的参考方法。批准标准-第2版M27-A2，国家临床实验室标准委员会，韦恩，PA，USA]。在供试植物中，45%（25种）对一种或多种供试真菌表现出抗真菌活性。最敏感的酵母菌是新型隐球菌，其次是克柔念珠菌，热带念珠菌和近平滑念珠菌。最不敏感的是白色念珠菌和光滑念珠菌。结果表明，不同来源的黄皮提取物具有较强的抗真菌活性，Sclerocariya birrea Sond、Turraea holstii Gurk、Sterculia africana（Lour）Fiori、Acacia robusta subsp. Usambarensis（Taub）Brenan、Cyphosterma hildebrandti（Gilg）、Desc、杜英Elaeodendron buchannanii（Lows）、阿拉伯金合欢Acacia nilotica（L.）Wild ex Del、多裂麻风树（Jatropha multifida L.）和蕨菜Pteridium aquilinum（L.）库恩(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Using the ethnobotanical approach, some Tanzanian plants reported to be used by traditional healers for the treatment of oral candidiasis and fungal infections of the skin were collected and screened for their antifungal activity against Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis, Candida krusei and Cryptococcus neoformans. A total of 65 crude methanol extracts belonging to 56 plant species and 38 families were screened using the broth microdilution method, according to the guidelines of the Clinical and Laboratory Standard Institute (CLSI) (formerly, National Committee for Clinical and Laboratory Standards) [National Committee for Clinical Laboratory Standards, 2002. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Approved Standard-2nd Edition M27-A2, National Committee for Clinical Laboratory Standards, Wayne, PA, USA]. Among the tested plant species, 45% (25 species) showed antifungal activity against one or more of the test fungi. The most susceptible yeasts were Cryptococcus neoformans, followed by Candida krusei, Candida tropicalis, and Candida parapsilosis. The least susceptible were Candida albicans and Candida glabrata. Strong antifungal activity was exhibited by extracts of Clausena anisata Oliv., Sclerocariya birrea Sond, Turraea holstii Gurk, Sterculia africana (Lour) Fiori, Acacia robusta subsp. Usambarensis (Taub) Brenan, Cyphosterma hildebrandti (Gilg), Desc, Elaeodendron buchannanii (Lows), Acacia nilotica (L.) Wild ex Del, Jatropha multifida L., and Pteridium aquilinum (L.) Kuhn. (c) 2006 Elsevier Ireland Ltd. All rights reserved.