Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis.
Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis.
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DOI：
10.1186/ar1754
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发表时间：
2005
影响因子：
4.9
通讯作者：
Vollmar B
中科院分区：
文献类型：
--
作者：
Gierer P;Ibrahim S;Mittlmeier T;Koczan D;Moeller S;Landes J;Gradl G;Vollmar B
更好地了解导致关节炎疾病的最初机制可以促进改进治疗策略的发展。我们研究了皮内注射牛胶原II型完全弗氏佐剂诱导关节炎(即胶原性关节炎[CIA])的敏感小鼠膝关节滑膜微循环。将敏感的DBA1/J和II型胶原T细胞受体转基因小鼠与CIA耐药的FVB/NJ小鼠进行比较。在关节炎临床症状出现之前，膝关节的活体荧光显微镜显示明显的白细胞激活，并与滑膜微血管的内皮衬里相互作用。这种最初的炎性细胞反应与疾病阶段的基因表达谱相关。在655个差异表达基因中，大多数属于参与细胞运动和结构、细胞周期和信号转导以及转录、蛋白质合成和代谢的基因。然而，已鉴定出24个黏附分子和趋化因子/细胞因子基因，其中一些已知与关节炎有关(例如CD44和中性粒细胞胞浆因子1)，另一些在这方面是新的(例如CC趋化因子配体27和IL-13受体α1)。关于滑膜组织微循环的在线活体数据，以及基因表达谱，强调了早期炎症事件在关节炎发展中所起的潜在作用。
A better understanding of the initial mechanisms that lead to arthritic disease could facilitate development of improved therapeutic strategies. We characterized the synovial microcirculation of knee joints in susceptible mouse strains undergoing intradermal immunization with bovine collagen II in complete Freund's adjuvant to induce arthritis (i.e. collagen-induced arthritis [CIA]). Susceptible DBA1/J and collagen II T-cell receptor transgenic mice were compared with CIA-resistant FVB/NJ mice. Before onset of clinical symptoms of arthritis, in vivo fluorescence microscopy of knee joints revealed marked leucocyte activation and interaction with the endothelial lining of synovial microvessels. This initial inflammatory cell response correlated with the gene expression profile at this disease stage. The majority of the 655 differentially expressed genes belonged to classes of genes that are involved in cell movement and structure, cell cycle and signal transduction, as well as transcription, protein synthesis and metabolism. However, 24 adhesion molecules and chemokine/cytokine genes were identified, some of which are known to contribute to arthritis (e.g. CD44 and neutrophil cytosolic factor 1) and some of which are novel in this respect (e.g. CC chemokine ligand-27 and IL-13 receptor α1). Online in vivo data on synovial tissue microcirculation, together with gene expression profiling, emphasize the potential role played by early inflammatory events in the development of arthritis.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
5.8
作者：
Wettenhall, JM;Smyth, GK
通讯作者：
Smyth, GK
影响因子：
14.9
作者：
Harris, MA;Clark, J;White, R
通讯作者：
White, R
影响因子：
6
作者：
Nandakumar, KS;Svensson, L;Holmdahl, R
通讯作者：
Holmdahl, R
影响因子：
--
作者：
Tak, PP;Taylor, PC;Maini, RN
通讯作者：
Maini, RN