Cytological evidence for chromosome elimination in wheat x Imperata cylindrica hybrids
Cytological evidence for chromosome elimination in wheat x Imperata cylindrica hybrids
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DOI：
10.1266/ggs.82.241
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发表时间：
2007-06-07
影响因子：
1.1
通讯作者：
Mukai, Yasuhiko
中科院分区：
文献类型：
--
作者：
Komeda, Norio;Chaudhar, Harinder K.;Mukai, Yasuhiko
利用白茅花粉诱导小麦单倍体是加倍育种的有效方法。研究了小麦与柱孢L. cylindrica杂交后产生单倍体的过程。我们对受精卵的细胞学观察表明，亲本配子成功受精。通过完全消除I.圆柱形染色体在受精卵第一次细胞分裂中观察到柱孢乳杆菌染色体的错分离。在分裂中期，柱孢L cylindrica的染色体没有聚集到赤道板上。在分裂后期，姐妹染色体不向两极移动，但它们的凝聚力正常释放。柱孢L cylindrica染色体在末期仍留在细胞质中，并从子核中消失。二细胞期以后，细胞核中已无柱状乳杆菌染色体，但细胞质中出现了含有柱状乳杆菌染色质的微核。这些观察结果表明，L cylindrica染色体是完全消除从细胞核中的第一次细胞分裂可能是由于缺乏功能的动粒。
Haploid induction of wheat by crossing with Imperata cylindrica pollen is an efficient method for doubled haploid breeding. We investigated the process of wheat haploid formation after crossing with L cylindrica. Our cytological observations of zygotes showed the successful fertilization of parental gametes. Wheat haploids were formed by complete elimination of I. cylindrica chromosomes. Missegregation of L cylindrica chromosomes was observed in the first cell division of zygote. At metaphase L cylindrica chromosomes did not congress onto the equatorial plate. The sister chromosomes did not move toward the poles during anaphase, though their cohesion was released normally. L cylindrica chromosomes were still in the cytoplasm at telophase and eliminated from daughter nuclei. After two-celled stage, we could find no L cylindrica chromosome in the nuclei but micronuclei containing L cylindrica chromatin in the cytoplasm. These observations indicate that L cylindrica chromosomes are completely eliminated from nuclei in the first cell division probably due to lack of functional kinetochores.