VASCULAR VOLUME IN B-16 ALLOGRAFTS AND HUMAN-MELANOMA XENOGRAFTS ESTIMATED BY MEANS OF HOECHST-33342
VASCULAR VOLUME IN B-16 ALLOGRAFTS AND HUMAN-MELANOMA XENOGRAFTS ESTIMATED BY MEANS OF HOECHST-33342
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DOI：
10.1002/path.1711570312
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发表时间：
1989-03-01
影响因子：
7.3
通讯作者：
FALLOWFIELD, ME
中科院分区：
文献类型：
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作者：
FALLOWFIELD, ME
用双苯甲胺荧光染料Hoechst 33342活体内染色观察了小鼠黑色素瘤B16和人黑色素瘤Mel-mo的血管构筑。在所研究的两条线的肿瘤边缘都有大量的毛细血管，但在结节内更稀少。用点计数法计算血管体积占总肿瘤体积的比例。在B16和Mel-mo中，血管体积百分比与对数肿瘤重量呈负相关。肿瘤坏死随着肿瘤大小的增加而增加，与血管体积百分比呈负相关，强调这些实验性肿瘤结节的中央灌注量不足。尽管肿瘤坏死的程度和模式不同，但在两个被检查的肿瘤中都可以看到这种灌注模式，在肿瘤结节周围有更高密度的功能性毛细血管。
The vasculature of B16, a murine melanoma and Mel-mo, a human melanoma, was studied using intravital staining of patent capillaries by the fluorescent bisbenzamine dye Hoechst 33342. Capillaries were numerous at the edge of tumors in both lines studied, but were scarcer within the nodules. Vascular volume as a proportion of total tumor volume was estiamted by means of point counting. In both B16 and Mel-mo, the percentage vascular volume was inversely related to log tumor weight. Tumor necrosis, which increased with tumor size, was inversely correlated with percentage vascular volume, emphasizing the central under-perfusion of these experimental tumor nodules. This pattern of perfusion, with greater density of functioning capillaries at the periphery of tumor nodules, was seen in both the tumor lines examined despite differences in the degree and pattern of necrosis.