A Chlamydomonas outer arm dynein mutant missing the alpha heavy chain.
A Chlamydomonas outer arm dynein mutant missing the alpha heavy chain.
复制标题
DOI：
10.1083/jcb.113.3.615
复制
发表时间：
1991-05
期刊：
影响因子：
--
通讯作者：
Kamiya R
中科院分区：
文献类型：
--
作者：
Sakakibara H;Mitchell DR;Kamiya R
分离到一种新的衣原体鞭毛突变体（oda-11），其缺失外臂动力蛋白的α重链，但保留β和γ重链。限制性片段长度多态性分析与α重链基因座基因组探针表明，oda-11突变与α重链的结构基因遗传连锁。在横截面电子显微照片中，oda-11轴丝缺乏外臂的最外附属物，表明α重链应该位于野生型外臂的该区域。以介于野生型（194微米/秒）和oda-1（缺失整个外动力蛋白臂的突变体）（62微米/秒）之间的中间速度。鞭毛拍频（约50 Hz）也介于野生型（约60 Hz）和oda-1（约26 Hz）之间。这些结果表明，衣原体的外动力蛋白臂可以在没有α重链的情况下组装，并且缺失α重链的外臂保留部分功能。
A novel Chlamydomonas flagellar mutant (oda-11) missing the alpha heavy chain of outer arm dynein but retaining the beta and gamma heavy chains was isolated. Restriction fragment length polymorphism analysis with an alpha heavy chain locus genomic probe indicated that the oda-11 mutation was genetically linked with the structural gene of the alpha heavy chain. In cross-section electron micrographs, the oda-11 axoneme lacked the outermost appendage of the outer arm, indicating that the alpha heavy chain should be located in this region in the wild-type outer arm. This mutant swam at 119 microns/s at 25 degrees C, i.e., at an intermediate speed between those of wild type (194 microns/s) and of oda-1 (62 microns/s), a mutant missing the entire outer dynein arm. The flagellar beat frequency (approximately 50 Hz) was also between those of wild type (approximately 60 Hz) and oda-1 (approximately 26 Hz). These results indicate that the outer dynein arm of Chlamydomonas can be assembled without the alpha heavy chain, and that the outer arm missing the alpha heavy chain retains partial function.