New crinine-type alkaloids with inhibitory effect on induction of inducible nitric oxide synthase from Crinum yemense
New crinine-type alkaloids with inhibitory effect on induction of inducible nitric oxide synthase from Crinum yemense
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DOI：
10.1021/np030529k
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发表时间：
2004-07-01
影响因子：
5.1
通讯作者：
Yoshikawa, M
中科院分区：
文献类型：
--
作者：
Abdel-Halim, OB;Morikawa, T;Yoshikawa, M
也门文殊兰球茎的 80% 甲醇水溶液提取物对脂多糖激活的巨噬细胞中一氧化氮的产生具有有效的抑制作用。从草药提取物中分离出三种新的克林宁型生物碱，即也门碱 A (1)、B (2) 和 C (3)，以及六种已知的生物碱。 1-3的绝对构型是根据化学和物理化学证据确定的。检查了分离的生物碱对脂多糖激活的巨噬细胞中一氧化氮产生的影响，并研究了几种生物碱，例如1, (+)-bulbispermine (6)、(+)-crinamine (7)、(+)-6-羟基crinamine (8) 和 (-)-lycorine (9) 对一氧化氮产生和诱导型一氧化氮合酶的诱导具有抑制作用。
The 80% aqueous methanolic extract from the bulbs of Crinum yemense showed a potent inhibitory effect on nitric oxide production in lipopolysaccharide-activated macrophages. Three new crinine-type alkaloids, yemenines A (1), B (2), and C (3), were isolated from the herbal extract together with six known alkaloids. The absolute configurations of 1-3 were determined on the basis of chemical and physicochemical evidence. The effects of the isolated alkaloids on nitric oxide production in lipopolysaccharide-activated macrophages were examined, and several alkaloids, e.g. 1, (+)-bulbispermine (6), (+)-crinamine (7), (+)-6-hydroxycrinamine (8), and (-)-lycorine (9), showed inhibitory effects on nitric oxide production and induction of inducible nitric oxide synthase.