Circularization of transduced fragments: a mechanism for adding segments to the bacterial chromosome.
Circularization of transduced fragments: a mechanism for adding segments to the bacterial chromosome.
复制标题
转导片段的环化：一种向细菌染色体添加片段的机制。
作者：
M. Schmid;J. Roth
具有直接顺序的冗余序列的广义转导片段可以在转导事件期间环化。所需冗余序列的长度至少可以短至IS10（1.4 kb）（Kleckner 1977）。环状转导片段能够与染色体中的同源序列重组。转导片段的环化和插入允许向细菌染色体添加片段，而不是像正常转导杂交中那样替换受体片段。它还提供了一种将细菌基因以任一方向易位到染色体上的各种特定位点的方法。这些事件的细菌进化的意义进行了讨论。
Generalized transducing fragments that have redundant sequences in direct order can circularize during transduction events. The length of the required redundant sequences can be at least as short as IS10 (1.4 kb) (Kleckner 1977). The circular transduced fragment is able to recombine with homologous sequences in the chromosome. Circularization and insertion of transduced fragments allow addition of segments to the bacterial chromosome rather than replacement of recipient segments as in a normal transductional cross. It also provides a method for translocation of bacterial genes to a variety of specific sites on the chromosome in either orientation. The significance of these events to bacterial evolution is discussed.