Ubiquitin is covalently attached to the p6Gag proteins of human immunodeficiency virus type 1 and simian immunodeficiency virus and to the p12Gag protein of Moloney murine leukemia virus
Ubiquitin is covalently attached to the p6Gag proteins of human immunodeficiency virus type 1 and simian immunodeficiency virus and to the p12Gag protein of Moloney murine leukemia virus
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DOI：
10.1128/jvi.72.4.2962-2968.1998
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发表时间：
1998-04-01
影响因子：
5.4
通讯作者：
Henderson, LE
中科院分区：
文献类型：
--
作者：
Ott, DE;Coren, LV;Henderson, LE
宿主蛋白在装配和出芽过程中被掺入逆转录病毒粒子中。我们已经检测了三种逆转录病毒，人类免疫缺陷病毒1型（HIV-1），猿猴免疫缺陷病毒（SIV）和莫洛尼鼠白血病病毒（Mo-MuLV），这些病毒粒子中每个病毒粒子内存在泛素。在蛋白酶处理以去除外部病毒以及污染的细胞蛋白质之后，分析病毒体内部剩余的蛋白质。此处呈现的结果显示，所有三种病毒体以病毒体中发现的Gag水平的约10%掺入泛素分子。除了游离泛素，共价泛素-Gag复合物被检测到，分离，并从所有三种病毒的特征。我们对处理过的病毒体的免疫印迹和蛋白质测序结果表明，约2%的HIV-1或SIV p6（Gag）共价连接到各自病毒体内的单个泛素分子上，Mo-MuLV病毒体中约2%至5%的p12（Gag）被单泛素化。这些结果表明，Gag的泛素化在这些逆转录病毒中是保守的，并且发生在Gag多聚蛋白的p6（Gag）部分，该区域可能参与组装和出芽。
Host proteins are incorporated into retroviral virions during assembly and budding, We have examined three retroviruses, human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus (SIV), and Moloney murine leukemia virus (Mo-MuLV), for the presence of ubiquitin inside each of these virions. After a protease treatment to remove exterior viral as well as contaminating cellular proteins, the proteins remaining inside the virion were analyzed, The results presented here show that all three virions incorporate ubiquitin molecules at approximately 10% of the level of Gag found in virions. In addition to free ubiquitin, covalent ubiquitin-Gag complexes were detected, isolated, and characterized from all three viruses. Our immunoblot and protein sequencing results on treated virions showed that approximately 2% of either HIV-1 or SIV p6(Gag) was covalently attached to a single ubiquitin molecule inside the respective,virions and that approximately 2 to 5% of the p12(Gag) in Mo-MuLV virions was monoubiquitinated. These results show that ubiquitination of Gag is conserved among these retroviruses and occurs in the p6(Gag) portion of the Gag polyprotein, a region that is likely to be involved in assembly and budding.