Androgen cytosol binding in exercise-induced sparing of muscle atrophy.
Androgen cytosol binding in exercise-induced sparing of muscle atrophy.
复制标题
雄激素胞质结合在运动引起的肌肉萎缩中的保留。
DOI：
10.1152/ajpendo.1984.247.5.e597
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
ChattertonJr,RT
中科院分区：
文献类型：
--
作者：
Hickson,RC;Kurowski,TT;Capaccio,JA;ChattertonJr,RT
这项研究的目的是确定运动引起的糖皮质激素引起的肌肉萎缩是否与雄激素胞质结合增加有关。雌性大鼠被分为久坐组或运动组，在跑步机上进行每天 100 分钟跑步训练，持续 13-15 周。在最后 12 天的训练中，每组又进一步细分为每日皮下注射醋酸可的松 (CA)（100 毫克/千克体重）或载体 1% 羧甲基纤维素的组。锻炼可以防止因腓肠肌和跖肌 CA 治疗而导致的 30-40% 的体重减轻。 Scatchard 对[3H]甲基三烯醇酮 (R1881)（一种与雄激素受体结合的合成雄激素）的特异性结合进行的分析，在经媒介物处理的久坐大鼠和训练过的大鼠的肌肉中呈非线性，并通过双组分结合模型解决了这一问题。线性斯卡查德图证明，归因于糖皮质激素受体的较低亲和力成分在糖皮质激素治疗的动物的肌肉中消失。所有治疗组的腓肠肌和跖肌中[3H]甲基三烯醇酮结合的雄激素成分的受体浓度相似。在腓肠肌的结合特异性研究中，在媒介物治疗组中，各种糖皮质激素和黄体酮对[3H]甲基三烯醇酮结合的相对较高的竞争通过CA治疗而减少。雄激素胞质受体水平没有变化表明，这并不是运动预防糖皮质激素引起的肌肉萎缩的机制。
This study was undertaken to determine whether the exercise-induced sparing of glucocorticoid-induced muscle atrophy is related to increased androgen cytosol binding. Female rats were divided into a sedentary or an exercise group that was trained by treadmill running 100 min/day for 13-15 wk. During the last 12 days of training, each of these groups was further subdivided into groups that received daily subcutaneous injections of cortisone acetate (CA) (100 mg/kg body wt) or the vehicle 1% carboxymethyl cellulose. Exercise prevented 30-40% of the weight loss due to CA treatment in gastrocnemius and plantaris muscles. Scatchard analyses of specific binding of [3H]methyltrienolone (R1881), a synthetic androgen that binds to androgen receptors, were nonlinear in muscles from vehicle-treated sedentary and trained rats and were resolved by a two-component binding model. The lower affinity component, which was attributed to a glucocorticoid receptor, disappeared in muscles of glucocorticoid-treated animals as evidenced by linear Scatchard plots. Receptor concentrations of the androgenic component of [3H]methyltrienolone binding were similar in gastrocnemius and plantaris muscles in all treatment groups. In binding specificity studies of gastrocnemius muscles, the relatively high competition by various glucocorticoids and progesterone for [3H]methyltrienolone binding in the vehicle-treated groups was reduced by CA treatment. The lack of change in androgen cytosol receptor levels suggests that this is not a mechanism by which exercise protects against glucocorticoid-induced muscle atrophy.