Phenotype and functionality of CD4+ and CD8+ T cells in the upper reproductive tract of healthy premenopausal women.
Phenotype and functionality of CD4+ and CD8+ T cells in the upper reproductive tract of healthy premenopausal women.
复制标题
健康绝经前女性上生殖道 CD4 和 CD8 T 细胞的表型和功能。
DOI：
10.1111/aji.12182
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Shacklett,BarbaraL
中科院分区：
文献类型：
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作者：
Shanmugasundaram,Uma;Critchfield,JWilliam;Pannell,Jane;Perry,Jean;Giudice,LindaC;Smith-McCune,Karen;Greenblatt,RuthM;Shacklett,BarbaraL
本研究的目的是调查健康绝经前妇女上生殖道中CD 4+和CD 8 +T细胞的表型和功能反应性。下生殖道经常被研究为性传播感染的部位;然而，上生殖道也可能是病原体（包括HIV-1）进入和传播的门户。研究方法23名健康女性在黄体中期采集子宫内膜活检、宫颈刮除、细胞刷和血液。T细胞分离和流式细胞仪分析。ResultsAs相比，他们的同行在血液中，子宫内膜和子宫颈内的T细胞有增强的CCR 5表达，并富集激活，效应记忆细胞。子宫内膜T细胞对多克隆刺激反应更敏感，产生广泛的细胞因子和趋化因子。ConclusionThese发现强调了子宫内膜T细胞对刺激的反应性，并揭示了它们的活化表型。这些发现也表明上生殖道对HIV-1感染的易感性。
ProblemThe goal of this study was to investigate the phenotype and functional responsiveness of CD4+and CD8+T‐cells in the upper reproductive tract of healthy premenopausal women. The lower reproductive tract is frequently studied as a site of sexually transmitted infections; however, the upper reproductive tract may also be a portal of entry and dissemination for pathogens, including HIV‐1.Method of StudyEndometrial biopsy, endocervical curettage, cytobrush, and blood were collected during mid‐luteal phase from 23 healthy women. T‐cells were isolated and analyzed by flow cytometry.ResultsAs compared with their counterparts in blood, endometrial and endocervical T‐cells had enhanced CCR5 expression, and were enriched for activated, effector memory cells. Endometrial T‐cells were more responsive to polyclonal stimuli, producing a broad range of cytokines and chemokines.ConclusionThese findings underscore the responsiveness of endometrial T‐cells to stimulation, and reveal their activated phenotype. These findings also suggest susceptibility of the upper reproductive tract to HIV‐1 infection.