Comprehensive behavioral phenotyping of calpastatin-knockout mice
Comprehensive behavioral phenotyping of calpastatin-knockout mice
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DOI：
10.1186/1756-6606-1-7
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发表时间：
2008-01-01
期刊：
影响因子：
3.6
通讯作者：
Saido, Takaomi C.
中科院分区：
文献类型：
--
作者：
Nakajima, Ryuichi;Takao, Keizo;Saido, Takaomi C.
背景：钙pastatin是细胞内钙活化蛋白酶calpain的内源性抑制剂。它被认为参与了长期可塑性和兴奋毒性途径的分子机制。然而，钙pastatin在体内的功能仍是未知的。为了检验calpastatin的生理作用，我们对calpastatin敲除小鼠进行了综合行为测试。结果：calpastatin敲除小鼠在应激环境下的运动活动下降，声惊吓反应下降，但我们观察到海马依赖记忆功能没有明显变化。结论：这些结果表明calpastatin可能与大脑功能的情感方面而不是认知方面更密切相关。
Background: Calpastatin is an endogenous inhibitor of calpain, intracellular calcium-activated protease. It has been suggested to be involved in molecular mechanisms of long-term plasticity and excitotoxic pathways. However, functions of calpastatin in vivo are still largely unknown. To examine the physiological roles of calpastatin, we subjected calpastatin-knockout mice to a comprehensive behavioral test battery.Results: Calpastatin-knockout mice showed decreased locomotor activity under stressful environments, and decreased acoustic startle response, but we observed no significant change in hippocampus-dependent memory function.Conclusion: These results suggest that calpastatin is likely to be more closely associated with affective rather than cognitive aspects of brain function.