A guide to the Michaelis-Menten equation: steady state and beyond
A guide to the Michaelis-Menten equation: steady state and beyond
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DOI：
10.1111/febs.16124
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发表时间：
2021-07-31
期刊：
影响因子：
5.4
通讯作者：
Srinivasan, Bharath
中科院分区：
文献类型：
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作者：
Srinivasan, Bharath
酶的现代定义与Leonor Michaelis和Maud Menten建立的Michaelis-Menten方程是同义词。大多数教科书或其中的章节，讨论酶学开始于在快速平衡(如Michaelis-Menten所做的)或稳态(如Briggs和Haldane修改)条件下的方程的推导，以强调该方程作为解释酶动力学结果所依赖的基石的重要性。然而，很少有教科书或专著努力将方程置于其正确的历史背景下，并讨论已进入其制度的假设。本指南将详细介绍这些内容。此外，本指南将试图灌输对数学曲线矩形双曲线、其独特属性以及曲线在生物系统中的普遍存在的鉴赏感。最后，本指南将讨论方程的局限性和它所体现的方法，并追踪研究人员如何试图超越稳态方法和米氏方程进入全进展曲线、稳态前和单周转动力学分析，以获得对酶动力学和催化的更深入的了解。
The modern definition of enzymology is synonymous with the Michaelis-Menten equation instituted by Leonor Michaelis and Maud Menten. Most textbooks, or chapters within, discussing enzymology start with the derivation of the equation under the assumption of rapid equilibrium (as done by Michaelis-Menten) or steady state (as modified by Briggs and Haldane) conditions to highlight the importance of this equation as the bedrock on which interpretation of enzyme kinetic results is dependent. However, few textbooks or monographs take the effort of placing the equation within its right historical context and discuss the assumptions that have gone into its institution. This guide will dwell on these in substantial detail. Further, this guide will attempt to instil a sense of appreciation for the mathematical curve rectangular hyperbola, its unique attributes and how ubiquitous the curve is in biological systems. To conclude, this guide will discuss the limitations of the equation, and the method it embodies, and trace the journey of how investigators are attempting to move beyond the steady-state approach and the Michaelis-Menten equation into full progress curve, pre-steady state and single-turnover kinetic analysis to obtain greater insights into enzyme kinetics and catalysis.