Design and synthesis of novel photoinduced electron transfer-based hybridization probes
Design and synthesis of novel photoinduced electron transfer-based hybridization probes
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新型光诱导电子转移杂交探针的设计与合成
DOI：
10.1016/j.bmc.2017.05.013
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发表时间：
2017
影响因子：
3.5
通讯作者：
Ueno Yoshihito
中科院分区：
文献类型：
--
作者：
Yokoyama Chika;Nakamoto Kosuke;Ueno Yoshihito
基于光诱导电子转移(PET)的杂交探针是一种用于DNA或RNA检测的线性、无猝灭剂的寡核苷酸(ON)探针。在这篇报道中，我们设计并合成了用于基于pET的杂交探针的新型腺苷类似物。特别是，含有哌嗪甲基的类似物在生理条件下表现出有效的猝灭性能。当含有类似物的探针与互补的DNA或RNA杂交时，荧光分别比单链状态增加了3倍或4倍。
Photoinduced electron transfer (PeT)-based hybridization probe is a linear and quencher-free oligonucleotide (ON) probe for DNA or RNA detection. In this report, we designed and synthesized novel adenosine analogues for PeT-based hybridization probe. In particular, the analogue containing a piperazinomethyl moiety showed effective quenching property under physiological conditions. When the probe containing the analogue was hybridized with a complementary DNA or RNA, the fluorescence increased 3- or 4-fold, respectively, compared to the single-stranded state.