A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA.
A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA.
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DOI：
10.1039/d1cc00016k
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发表时间：
2021-02
影响因子：
4.9
通讯作者：
Bingyao Wang;Yi Chen;Xiong Zhang;Zhuoran Jiang;Yafen Wang;Kun Chen;F. Wang;X. Weng;Xiang Zhou
中科院分区：
文献类型：
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作者：
Bingyao Wang;Yi Chen;Xiong Zhang;Zhuoran Jiang;Yafen Wang;Kun Chen;F. Wang;X. Weng;Xiang Zhou
我们报道了一种结合UNG切除和荧光探针标记的基因组DNA脱氧尿嘧啶检测新方法。UNG可以去除尿嘧啶碱基，引入脱碱基位点，由于PET效应的抑制，脱碱基位点可以与NRNO反应产生强烈的荧光。它还可以导致聚合酶延伸停止，以提供dU位点信息的细节。
We report a new method for dU detection in genomic DNA combined with UNG excision and fluorescent probe labeling. UNG can remove uracil bases to introduce abasic sites, which can react with NRNO to produce intense fluorescence because of the inhibition of the PET effect. It can also cause the polymerase extension to stop to provide details of dU site information.