Clinical significance of detecting circulating tumor cells in colorectal cancer using subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH).
Clinical significance of detecting circulating tumor cells in colorectal cancer using subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH).
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消减富集和免疫染色荧光原位杂交（SE-iFISH）检测结直肠癌循环肿瘤细胞的临床意义
DOI：
10.18632/oncotarget.15452
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发表时间：
2017-03-28
期刊：
影响因子：
--
通讯作者：
Zhang W
中科院分区：
文献类型：
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作者：
Wu W;Zhang Z;Gao XH;Shen Z;Jing Y;Lu H;Li H;Yang X;Cui X;Li Y;Lou Z;Liu P;Zhang C;Zhang W
循环肿瘤细胞（CTC）可用于结直肠癌的早期检测。本研究描述了一种新开发的平台，集成减法富集和免疫染色-荧光原位杂交（SE-iFISH），以评估结直肠癌中的CTC。SE-iFISH检测44例术前结直肠癌患者中的40例，敏感性为90.9%，显著高于CellSearch系统（90.9% vs. 43.2%，P=0.033）。肿瘤分期、生存率和术前CTC数之间无显著相关性。10名结直肠癌患者在手术后一周检测到了CTC; 7名CTC数量减少（与术前CTC数量相比）的患者没有复发;而3名CTC数量增加的患者中有2名出现肿瘤复发。此外，在34例结直肠癌患者中，术后3个月检测到CTC; CTC =2的患者<2 at three months after surgery had significantly longer Progression Free Survival than those with CTC>（P=0.019）; CTC数量减少的患者（与术前CTC数量相比）的无进展生存期明显长于CTC数量增加的患者（P=0.003）。总之，使用SE-iFISH可以在结直肠癌的各个阶段检测到CTC。动态监测CTC数量可预测复发和预后。
Circulating tumor cells (CTC) are useful in early detection of colorectal cancer. This study described a newly developed platform, integrated subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH), to assess CTCs in colorectal cancer. CTCs were detected by SE-iFISH in 40 of 44 preoperative colorectal cancer patients, and yielded a sensitivity of 90.9%, which was significantly higher than CellSearch system (90.9% vs. 43.2%, P=0.033). No significant association was found between tumor stage, survival and preoperative CTC number. CTCs were detected in 10 colorectal cancer patients one week after surgery; seven patients with decreased CTC numbers (compared with preoperative CTC number) were free of recurrence; whereas two of the three patients with increased CTC numbers had tumor recurrence. Moreover, CTCs were detected in 34 colorectal cancer patients three months after surgery; patients with CTC<2 at three months after surgery had significantly longer Progression Free Survival than those with CTC>=2 (P=0.019); patients with decreased CTC number (compared with preoperative CTC number) had significantly longer Progression Free Survival than those with increased CTC number (P=0.003). In conclusion, CTCs could be detected in various stages of colorectal cancer using SE-iFISH. Dynamic monitoring of CTC numbers could predict recurrence and prognosis.