High-Resolution Mass Spectrometers
High-Resolution Mass Spectrometers
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DOI：
10.1146/annurev.anchem.1.031207.112945
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发表时间：
2008-01-01
影响因子：
8
通讯作者：
Hendrickson, Christopher. L.
中科院分区：
文献类型：
--
作者：
Marshall, Alan G.;Hendrickson, Christopher. L.
在过去的十年中，质谱仪已经彻底改变了越来越高的质量分辨率的仪器。对于高达400 Da的小分子（例如，药物，代谢物和各种天然有机混合物，从食物到石油），有可能确定元素组成（CcHhNnOoSsPp.）通过精确的质量测量同时分析数千种化学成分（如果包括额外的信息，可以在高达1000 Da的范围内进行同样的测量）。在更高的质量，它成为可能的蛋白质（包括翻译后修饰）从蛋白水解肽，以及脂质，糖缀合物，和其他生物成分。在甚至更高的质量（类似于100，000 Da或更高）下，可以表征完整蛋白质的翻译后修饰，并绘制大生物分子复合物的结合表面。在这里，我们回顾了最高分辨率的分析质谱仪（飞行时间和傅立叶变换离子回旋共振和轨道阱质量分析仪）的原理和技术，并描述了一些代表性的高分辨率应用。
Over the past decade, mass spectrometry has been revolutionized by access to instruments of increasingly high mass-resolving power. For small molecules up to similar to 400 Da (e.g., drugs, metabolites, and various natural organic mixtures ranging from foods to petroleum), it is possible to determine elemental compositions (CcHhNnOoSsPp ...) of thousands of chemical components simultaneously from accurate mass measurements (the same can be done up to 1000 Da if additional information is included). At higher mass, it becomes possible to identify proteins (including posttranslational modifications) from proteolytic peptides, as well as lipids, glycoconjugates, and other biological components. At even higher mass (similar to 100,000 Da or higher), it is possible to characterize posttranslational modifications of intact proteins and to map the binding surfaces of large biomolecular complexes. Here we review the principles and techniques of the highest-resolution analytical mass spectrometers (time-of-flight and Fourier transform ion cyclotron resonance and orbitrap mass analyzers) and describe some representative high-resolution applications.