Impact of Local Drug Delivery of Minocycline on the Subgingival Microbiota during Supportive Periodontal Therapy: A Randomized Controlled Pilot Study.
Impact of Local Drug Delivery of Minocycline on the Subgingival Microbiota during Supportive Periodontal Therapy: A Randomized Controlled Pilot Study.
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DOI：
10.3390/dj8040123
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发表时间：
2020-10-27
影响因子：
2.6
通讯作者：
Yamazaki K
中科院分区：
文献类型：
--
作者：
Miyazawa H;Nakajima T;Horimizu M;Okuda K;Sugita N;Yamazaki K;Li L;Hayashi-Okada Y;Arita T;Nishimoto M;Nishida M;Genco RJ;Yamazaki K
本研究的目的是检查辅助局部米诺环素给药对残余牙周袋龈下菌斑样本微生物参数的影响。招募了十名接受支持性牙周治疗方案的慢性牙周炎患者。龈下清创后，每周一次向选定部位施用 2% 米诺环素凝胶 Periocline™（测试组）或安慰剂（对照组），持续三周。在基线、第 4 周和第 8 周时收集龈下菌斑。通过16S核糖体RNA测序分析微生物组成。在测试组中，α-多样性（均匀度）与基线相比有所下降（p = 0.005），并且在四周时与对照组相比较低（p = 0.003）。第 4 周时，两组之间的微生物群落组成存在显着差异 (p = 0.029)。这些变化可归因于与牙周炎相关的细菌减少和与牙周健康相关的细菌增加。此外，探诊出血的改善持续到八周；然而，在八周时观察到 2% 米诺环素凝胶几乎没有对微生物产生影响。在整个八周的实验期间，对照组没有表现出任何变化。因此，局部给予米诺环素可以改变残留牙周袋的龈下微生物群落。
The aim of this study was to examine the effect of adjunct local minocycline administration on the microbiological parameters of subgingival plaque samples in the residual periodontal pockets. Ten chronic periodontitis patients under a supportive periodontal therapy regimen were recruited. After subgingival debridement, either 2% minocycline gel, Periocline™, (Test Group) or a placebo (Control Group) was administered to the selected sites once a week for three weeks. Subgingival plaque was collected at baseline, and at four weeks and eight weeks. The microbiological composition was analyzed by 16S ribosomal RNA sequencing. In the Test Group, α-diversity (evenness) decreased compared to the baseline (p = 0.005) and was lower compared to the control group at four weeks (p = 0.003). The microbial community composition between the two groups was significantly different at four weeks (p = 0.029). These changes were attributable to a decrease in the bacteria associated with periodontitis and an increase in the bacteria associated with periodontal health. Additionally, the improvement in bleeding on probing continued at eight weeks; however, there were little microbial effects of 2% minocycline gel observed at eight weeks. The control group demonstrated no change throughout the eight-week experimental period. Thus, local minocycline administration can change the subgingival microbial community of residual periodontal pockets.
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影响因子：
3.7
作者：
Jünemann S;Prior K;Szczepanowski R;Harks I;Ehmke B;Goesmann A;Stoye J;Harmsen D
通讯作者：
Harmsen D
Keystone-Pathogen假设。
DOI：
10.1038/nrmicro2873
发表时间：
2012-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.5
作者：
Mdala I;Olsen I;Haffajee AD;Socransky SS;de Blasio BF;Thoresen M
通讯作者：
Thoresen M
影响因子：
6.7
作者：
Matuliene, Giedre;Pjetursson, Bjarni E.;Lang, Niklaus P.
通讯作者：
Lang, Niklaus P.
影响因子：
2.1
作者：
Campanile, Veronique S. Mueller;Giannopoulou, Catherine;Mombelli, Andrea
通讯作者：
Mombelli, Andrea