Suprathreshold auditory cortex activation visualized by intrinsic signal optical imaging
Suprathreshold auditory cortex activation visualized by intrinsic signal optical imaging
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DOI：
10.1093/cercor/6.2.120
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发表时间：
1996-03-01
期刊：
影响因子：
3.7
通讯作者：
Frostig, RD
中科院分区：
文献类型：
--
作者：
Bakin, JS;Kwon, MC;Frostig, RD
本实验利用内信号光学成像技术研究了大鼠和豚鼠听皮层的阈上音调组织结构。光学成像显示，阈上纯正弦波音调刺激（25-80分贝）诱发活动的大皮层地区的tonotopically组织。激活区域的三维表面图显示了由许多局部峰和谷组成的“斑片状”神经诱发活动。随后在相同的主题详细的电生理映射证实了本地化的神经诱发活动的基础上，光学成像，包括响应测试频率在皮层位点超过2个八度远离阈值定义的isobutes轮廓。这种技术的成功可视化听觉皮层功能组织在阈上刺激水平将允许未来的调查听觉皮层的频率表示，包括表征可塑性诱导的各种实验操作。
The suprathreshold tonotopic organization of rat and guinea pig auditory cortex was investigated using intrinsic signal optical imaging through a thinned skull. Optical imaging revealed that suprathreshold pure sine wave tone stimulation (25-80 dB) evoked activity over large cortical areas that were tonotopically organized. Three-dimensional surface plots of the activated areas revealed ''patchy'' auditory-evoked activity consisting of numerous local peaks and valleys building to a maximum. Subsequent detailed electrophysiological mapping in the same subjects confirmed the localization of auditory-evoked activity based on optical imaging, including responses to a test frequency at cortical loci more than 2 octaves away from the threshold-defined isofrequency contour. The success of this technique in visualizing auditory cortex functional organization at suprathreshold stimulus levels will allow for future investigations of auditory cortex frequency representation, including representational plasticity induced by a variety of experimental manipulations.