iTRAQ protein profile analysis of Citrus sinensis roots in response to long-term boron-deficiency.
iTRAQ protein profile analysis of Citrus sinensis roots in response to long-term boron-deficiency.
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DOI：
10.1016/j.jprot.2013.04.025
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发表时间：
2013-11
影响因子：
3.3
通讯作者：
Lintong Yang;Y. Qi;Yi-Bin Lu;Peng Guo;W. Sang;H. Feng;Hong-Xing Zhang;Lisong Chen
中科院分区：
文献类型：
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作者：
Lintong Yang;Y. Qi;Yi-Bin Lu;Peng Guo;W. Sang;H. Feng;Hong-Xing Zhang;Lisong Chen
用缺硼（0 μM H3BO3）或足硼（10 μM H3BO3）营养液对柑橘幼苗进行15周的施肥。随后，采用iTRAQ分析比较了缺b根和足b根中蛋白质的丰度。在缺b根中，鉴定出164个上调蛋白和225个下调蛋白。这些蛋白质的功能可分为以下几类：蛋白质代谢、核酸代谢、应激反应、碳水化合物和能量代谢、细胞转运、细胞壁和细胞骨架代谢、生物调节和信号转导以及脂质代谢。根系对缺b的适应性反应可能包括以下几个方面：(a)根系呼吸减少；(b)提高清除活性氧（ROS）的总能力；(c)增强细胞运输。iTRAQ鉴定的差异表达蛋白比2D凝胶电泳检测到的差异表达蛋白要大得多，并且在这项工作中鉴定了许多涉及细胞运输、生物调节和信号转导、应激反应等代谢过程的新的b缺陷反应蛋白。我们的研究结果表明柑橘根具有显著的代谢灵活性，这可能有助于缺b植物的存活。这代表了对b缺乏反应的蛋白质谱的最全面的分析。在这项研究中，我们发现了许多新的蛋白质，这些蛋白质参与细胞运输、生物调节和信号转导、应激反应和其他代谢过程，这些蛋白质以前并不知道与根b缺乏反应有关。因此，我们的论文代表了对b缺乏反应的蛋白质谱的最全面的分析，并为植物对b缺乏的反应提供了新的信息。这篇文章是《翻译植物蛋白质组学》特刊的一部分。
Seedlings ofCitrus sinensiswere fertilized with boron (B)-deficient (0 μM H3BO3) or -sufficient (10 μM H3BO3) nutrient solution for 15 weeks. Thereafter, iTRAQ analysis was employed to compare the abundances of proteins from B-deficient and -sufficient roots. In B-deficient roots, 164 up-regulated and 225 down-regulated proteins were identified. These proteins were grouped into the following functional categories: protein metabolism, nucleic acid metabolism, stress responses, carbohydrate and energy metabolism, cell transport, cell wall and cytoskeleton metabolism, biological regulation and signal transduction, and lipid metabolism. The adaptive responses of roots to B-deficiency might include following several aspects: (a) decreasing root respiration; (b) improving the total ability to scavenge reactive oxygen species (ROS); and (c) enhancing cell transport. The differentially expressed proteins identified by iTRAQ are much larger than those detected using 2D gel electrophoresis, and many novel B-deficiency-responsive proteins involved in cell transport, biological regulation and signal transduction, stress responses and other metabolic processes were identified in this work. Our results indicate remarkable metabolic flexibility of citrus roots, which may contribute to the survival of B-deficient plants. This represents the most comprehensive analysis of protein profiles in response to B-deficiency.Biological significanceIn this study, we identified many new proteins involved in cell transport, biological regulation and signal transduction, stress responses and other metabolic processes that were not previously known to be associated with root B-deficiency responses. Therefore, our manuscript represents the most comprehensive analysis of protein profiles in response to B-deficiency and provides new information about the plant response to B-deficiency.This article is part of a Special Issue entitled: Translational Plant Proteomics.