Significance of(1,3)-β-D-Glucan Detection in Three Kinds of Fluid Samples from Rat Model of Systemic Trichosporosis
Significance of(1,3)-β-D-Glucan Detection in Three Kinds of Fluid Samples from Rat Model of Systemic Trichosporosis
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发表时间：
2007
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通讯作者：
Wang Cong-min
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作者：
Wang Cong-min
目的观察不同体液样本中（1，3）-β-D-葡聚糖含量对系统性毛孢子菌病大鼠模型的意义。方法建立系统性毛孢子菌病动物模型，收集支气管肺泡灌洗液（BALF）、血浆和尿液样本进行检测，并进行G-试验、巢式PCR和真菌培养检查。结果感染早期（10 d），血浆、血浆和尿液样本G-试验敏感性最高。30例血浆标本中，G试验和巢式PCR血真菌培养的平均敏感性分别为76.76%、68.85%和3.33%，经统计学分析，G试验和巢式PCR血真菌培养的平均敏感性分别为76.76%、68.85%和3.33%，BALF和尿液的平均敏感性分别为80.00%、86.67%和6.67%，与对照组比较差异有显著性。G-试验敏感性与真菌培养有显著性差异血浆中（1，3）-β-D-葡聚糖浓度在感染初期升高，随病情好转而降低，结论初步结果提示，检测部分体液中的（1，3）-β-D-葡聚糖有助于系统性毛孢子菌病的早期诊断、判断感染范围及预后。
OBJECTIVE To observe the significance of(1,3)-β-D-glucan in different humor samples on the rat model of systemic trichosporosis.METHODS Established the animal model with systemic infection of trichosporosis,to collect the bronchoalveolar lavage fluid(BALF),plasma and urine samples for determining,and checked with G-test,nested PCR and fungus culture.RESULTS In the earlier infection(10d),the sensitivity of G-test in the samples of plasma,BALF and urine were 80.00%,86.67%,and 6.67%,respectively,and had significant difference with control group.In the 30 plasma samples,the average sensitivity of G-test and nested-PCR blood fungus culture was 76.76%,68.85%,and 3.33%,respectively.With the statistical analysis,the sensitivity of G-test had significant deviation than fungus culture(P0.05).The concentration of(1,3)-β-D-glucan in the plasma increased at the initial infected stage and decreased as these rats got well,and reflected linear correlation with the number of infected viscera organs(P0.05).CONCLUSIONS The primary results suggested that the(1,3)-β-D-glucan detection in some fluid samples help to make early diagnosis,and evaluate the infection range and the prognosis of the systemic trichosporosis.