Iron-Catalysed Radical Polymerisation by Living Bacteria
Iron-Catalysed Radical Polymerisation by Living Bacteria
复制标题
活细菌的铁催化自由基聚合
DOI：
10.1002/ange.201915084
复制
发表时间：
2020
影响因子：
--
通讯作者：
Bennett M
中科院分区：
文献类型：
--
作者：
Bennett M
利用细胞氧化还原过程进行非生物合成的能力可能使工程杂交生命系统的制备成为可能。为了实现这一目标，我们描述了一种新的细菌介导的铁催化可逆失活自由基聚合（RDRP），具有一系列金属螯合剂和单体，可在环境条件下与细菌氧化还原起始步骤一起使用以生成聚合物。选择金属铜球菌、大肠杆菌和产孢梭菌的氧化还原酶系统，并评估它们诱导聚合物形成的能力。考察了电池和催化剂浓度、引发剂种类和单体类型等参数。水溶性合成聚合物是在细菌存在下产生的，充分保存了细胞活力。这种方法提供了一种方法，通过这种方法，细菌氧化还原系统可以在“宿主”细胞存在的情况下产生“非自然”聚合物，从而建立了制造自然合成杂化结构和共轭物的可能性。
The ability to harness cellular redox processes for abiotic synthesis might allow the preparation of engineered hybrid living systems. Towards this goal we describe a new bacteria‐mediated iron‐catalysed reversible deactivation radical polymerisation (RDRP), with a range of metal‐chelating agents and monomers that can be used under ambient conditions with a bacterial redox initiation step to generate polymers.Cupriavidus metallidurans,Escherichia coli, andClostridium sporogenesspecies were chosen for their redox enzyme systems and evaluated for their ability to induce polymer formation. Parameters including cell and catalyst concentration, initiator species, and monomer type were investigated. Water‐soluble synthetic polymers were produced in the presence of the bacteria with full preservation of cell viability. This method provides a means by which bacterial redox systems can be exploited to generate “unnatural” polymers in the presence of “host” cells, thus setting up the possibility of making natural–synthetic hybrid structures and conjugates.