Experience-dependent plasticity of dendritic spines in the developing rat barrel cortex in vivo
Experience-dependent plasticity of dendritic spines in the developing rat barrel cortex in vivo
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DOI：
10.1038/35009107
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发表时间：
2000-04-20
期刊：
影响因子：
64.8
通讯作者：
Svoboda, K
中科院分区：
文献类型：
--
作者：
Lendvai, B;Stern, EA;Svoboda, K
神经元结构的变化是皮层可塑性的基础吗（1，2）？在这里，我们使用发育中的大鼠桶状皮质（5）第2/3层锥体神经元的延时双光子显微镜（3，4）来成像树突棘和丝状伪足的结构动力学。我们发现这些突起是高度能动的：棘和丝状伪足出现，消失或改变形状超过几十分钟。为了测试感官体验是否驱动这种运动性，我们在成像前一到三天修剪了胡须。在出生后第11-13天（P）的关键时期，感觉剥夺显著（约40%）降低了桶皮质被剥夺区域的蠕动，但对年轻（P8-10）或老年（P14-16）动物没有影响。出乎意料的是，胡须修剪并没有改变棘和丝状伪足的密度、长度或形状。然而，在关键期的感觉剥夺降低了2/3层感受野的调谐。因此，感觉经验驱动树突的结构可塑性，这可能是神经回路重组的基础。
Do changes in neuronal structure underlie cortical plasticity(1,2)? Here we used time-lapse two-photon microscopy(3,4) of pyramidal neurons in layer 2/3 of developing rat barrel cortex(5) to image the structural dynamics of dendritic spines and filopodia. We found that these protrusions were highly motile: spines and filopodia appeared, disappeared or changed shape over tens of minutes. To test whether sensory experience drives this motility we trimmed whiskers one to three days before imaging. Sensory deprivation markedly (similar to 40%) reduced protrusive motility in deprived regions of the barrel cortex during a critical period around postnatal days (P)11-13, but had no effect in younger (P8-10) or older (P14-16) animals. Unexpectedly, whisker trimming did not change the density, length or shape of spines and filopodia. However, sensory deprivation during the critical period degraded the tuning of layer 2/3 receptive fields. Thus sensory experience drives structural plasticity in dendrites, which may underlie the reorganization of neural circuits.