Whole-cell recordings and photolysis of caged compounds in olfactory sensory neurons isolated from the mouse
Whole-cell recordings and photolysis of caged compounds in olfactory sensory neurons isolated from the mouse
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DOI：
10.1093/chemse/bjg063
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发表时间：
2003-10-01
期刊：
影响因子：
3.5
通讯作者：
Menini, A
中科院分区：
文献类型：
--
作者：
Lagostena, L;Menini, A
基因操作和分子生物学技术在小鼠嗅觉研究中已经得到了很好的发展，而小鼠嗅觉感觉神经元的电生理特性还没有得到广泛的研究。电压门控电流由瞬时内向电流和瞬时外向电流组成。在所测试的嗅觉感觉神经元中，1 - 2%的细胞对有气味的桉叶素产生内向电流。笼状化合物被引入到细胞质中，通过贴片移液管和闪光光解的笼状环核苷酸激活的内向电流在94%的细胞。当闪光灯照射纤毛时，反应潜伏期、上升时间和持续时间均短于照射索马时。光解反应的幅度取决于光强度，并且该关系由Hill方程拟合，Hill系数为3.2。这些结果表明，它是可能的，以获得记录在全细胞配置从小鼠分离的嗅觉感觉神经元和电压门控电流和转导特性在很大程度上是类似的两栖动物。
Gene manipulation and molecular biological techniques for the study of olfaction are well developed in mice, while electrophysiological properties of mouse olfactory sensory neurons have been less extensively investigated, We used the whole-cell voltage-clamp technique in mouse isolated olfactory sensory neurons to investigate both voltage-gated and transduction currents. Voltage-gated currents were composed of transient inward currents followed by outward currents with transient and sustained components. Of the tested olfactory sensory neurons,1 2% responded to the odorant cineole with an inward current. Caged compounds were introduced into the cytoplasm through the patch pipette and flash photolysis of caged cyclic nucleotides activated an inward current in 94% of the cells. When the flash was localized at the cilia, the response latency, rising time and duration were shorter than when the flash illuminated the soma. The amplitude of the photolysis response was dependent on light intensity and the relation was fitted by the Hill equation, with a Hill coefficient of 3.2. These results demonstrate that it is possible to obtain recordings in the whole-cell configuration from olfactory sensory neurons isolated from the mouse and that voltage-gated currents and transduction properties are largely similar to those of amphibians.