PARK7 and nucleoside diphosphate kinase A as plasma markers for the early diagnosis of stroke
PARK7 and nucleoside diphosphate kinase A as plasma markers for the early diagnosis of stroke
复制标题
DOI：
10.1373/clinchem.2005.053942
复制
发表时间：
2005-11-01
影响因子：
9.3
通讯作者：
Sanchez, JC
中科院分区：
文献类型：
--
作者：
Allard, L;Burkhard, PR;Sanchez, JC
背景：脑卒中的血浆标志物可用于诊断和预后以及预测脑卒中患者对治疗的反应。PARK 7和核苷二磷酸激酶A（NDKA）在人死后脑脊液（CSF）（一种全脑损伤的模型）中增加，这表明在CSF中的测量，更重要的是，在血浆中的测量可用作中风的生物标志物。我们在3项独立的欧洲和北美回顾性研究中使用ELISA测量血浆中的PARK 7和NDKA，这些研究共包括622名中风患者，结果：PARK 7和NDKA的敏感性分别为54%-91%和70%-90%，特异性分别为80%-97%和90%-97%。结论：PARK 7和NDKA可能是脑卒中早期诊断的有用的血浆生物标志物。此外，本研究证明了死后CSF蛋白分析作为发现缺血性脑损伤血浆标志物的第一步的实用性。(c)2005年美国临床化学协会。
Background: Plasma markers for stroke could be useful in diagnosis and prognosis and in prediction of response of stroke patients to therapy. PARK7 and nucleoside diphosphate kinase A (NDKA) are increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke.Methods: We used ELISA to measure PARK7 and NDKA in plasma in 3 independent European and North American retrospective studies encompassing a total of 622 stroke patients and 165 control individuals.Results: Increases in both biomarkers were highly significant, with sensitivities of 54%-91% for PARK7 and 70%-90% for NDKA and specificities of 80%-97% for PARK7 and 90%-97% for NDKA. The concentrations of both biomarkers increased within 3 h of stroke onset.Conclusions: PARK7 and NDKA may be useful plasma biomarkers for the early diagnosis of stroke. In addition, this study demonstrated the utility of analysis of postmortem CSF proteins as a first step in the discovery of plasma markers of ischemic brain injury. (c) 2005 American Association for Clinical Chemistry.