Antigenic glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. IV. Immunogenic properties of separated haemagglutinin glycopolypeptides.
Antigenic glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. IV. Immunogenic properties of separated haemagglutinin glycopolypeptides.
复制标题
流感病毒血凝素的抗原性糖多肽HA1和HA2。
作者：
B. Styk;G. Russ;K. Polakova
高纯度的血凝素糖多肽HA1和HA2能有效地诱导抗体反应。HA2的免疫原性明显高于HA1。在凝胶双向免疫扩散实验中，HA2免疫兔血清比HA1免疫兔血清产生更明显的沉淀线。两种兔血清均与同源抗原和菠萝酶释放纯化的血凝素(B-HA)沉淀。在放射免疫分析中，用HA2免疫的兔血清可与125I标记的HA2结合，并优先与125I标记的HA2反应。一般来说，用HA1免疫的兔血清对125I标记的HA1结合效价很低：通常它们也能与125I标记的B-HA和125I标记的HA2反应。只有在短时间间隔注射少量HA1的兔才能优先结合~(125)I标记HA1。糖多肽HA1和HA2不能诱导兔产生血凝抑制抗体和病毒中和抗体。
Highly purified haemagglutinin glycopolypeptides HA1 and HA2 were effective in eliciting an antibody response. HA2 had a markedly greater immunogenic potential than HA1. In gel double immunodiffusion, sera from rabbits immunized with HA2 produced more distinct precipitin lines than sera obtained by immunization with HA1. Both kinds of rabbit sera gave precipitation with homologous antigen and with bromelain-released and purified haemagglutinin (B-HA). In radioimmunoassay, sera from rabbits immunized with HA2 revealed considerable titres for 125I-labelled HA2 binding and reacted preferentially with 125I-labelled HA2. In general, sera from rabbits immunized with HA1 exhibited low titres for 125I-labelled HA1 binding: usually they reacted also with 125I-labelled B-HA and 125I-labelled HA2. Only rabbits injected with a few doses of HA1 at short intervals revealed preferential binding for 125I-labelled HA1. Glycopolypeptides HA1 and HA2 failed to induce haemagglutination-inhibiting and virus neutralizing antibodies in rabbits.