Assembly of bacteriophage 80α capsids in a Staphylococcus aureus expression system.
Assembly of bacteriophage 80α capsids in a Staphylococcus aureus expression system.
复制标题
金黄色葡萄球菌表达系统中噬菌体 80α 衣壳的组装。
DOI：
10.1016/j.virol.2012.08.031
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Dokland,Terje
中科院分区：
文献类型：
--
作者：
Spilman,MichaelS;Damle,PriyadarshanK;Dearborn,AltairaD;Rodenburg,CynthiaM;Chang,JennyR;Wall,ErinA;Christie,GailE;Dokland,Terje
80DNA是金黄色葡萄球菌的一种温和的双链α噬菌体，可以作为动员金黄色葡萄球菌致病岛(包括SAPI)的帮手。当SAPI被80α动员时，SAPI基因组被包装成由噬菌体蛋白组成的衣壳，但比正常情况下由噬菌体形成的衣壳要小。这种大小的决定取决于SaPI1蛋白CPMA和CpmB。在这里，我们证明了80α衣壳和支架蛋白在金黄色葡萄球菌中共表达，但在大肠杆菌中不表达，导致了原衣壳相关结构的形成，这表明组装需要一个宿主辅助因子。衣壳和支架蛋白在金黄色葡萄球菌中表达后，也会经历正常的N-末端处理，这意味着宿主蛋白酶。我们还发现，在金黄色葡萄球菌中，SaPI1蛋白Cpma和CpmB与80α衣壳蛋白和支架蛋白共表达时，促进了小衣壳的形成。
80α is a temperate, double-stranded DNA bacteriophage of Staphylococcus aureus that can act as a “helper” for the mobilization of S. aureus pathogenicity islands (SaPIs), including SaPI1. When SaPI1 is mobilized by 80α, the SaPI genomes are packaged into capsids that are composed of phage proteins, but that are smaller than those normally formed by the phage. This size determination is dependent on SaPI1 proteins CpmA and CpmB. Here, we show that co-expression of the 80α capsid and scaffolding proteins in S. aureus, but not in E. coli, leads to the formation of procapsid-related structures, suggesting that a host co-factor is required for assembly. The capsid and scaffolding proteins also undergo normal N-terminal processing upon expression in S. aureus, implicating a host protease. We also find that SaPI1 proteins CpmA and CpmB promote the formation of small capsids upon co-expression with 80α capsid and scaffolding proteins in S. aureus.