Comparison of adhesive gut bacteria composition, immunity, and disease resistance in juvenile hybrid tilapia fed two different Lactobacillus strains
Comparison of adhesive gut bacteria composition, immunity, and disease resistance in juvenile hybrid tilapia fed two different Lactobacillus strains
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饲喂两种不同乳酸菌菌株的杂交罗非鱼幼鱼粘附肠道细菌组成、免疫力和抗病性的比较
DOI：
10.1016/j.fsi.2013.04.010
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发表时间：
2013-07-01
影响因子：
4.7
通讯作者：
Zhou, Zhigang
中科院分区：
文献类型：
--
作者：
Liu, Wenshu;Ren, Pengfei;Zhou, Zhigang
本研究比较了两种乳酸杆菌菌株，即高粘附性短乳杆菌JCM 1170（HALB）和低粘附性嗜酸乳杆菌JCM 1132（LALB）对杂交罗非鱼幼鱼的存活和生长、肠道粘附菌群、免疫力和抗病原菌感染的影响。在5周的饲养试验期间，给鱼喂食含有0至10（9）个细胞/g饲料的两种乳杆菌菌株的饮食。在开始时和第10、20和35天采集肠、肾和脾样品，用于分析应激耐受性和细胞因子基因mRNA水平，并评估粘附性肠道细菌群落的多样性。在饲喂试验后，还进行了14天的嗜水气单胞菌NJ-1浸泡攻击。结果表明，在不同的饮食处理的存活率，体重增加，或饲料转化率没有显着差异。在喂食HALB或LALB的鱼中，粘附性肠道细菌群落发生了显著改变，但对粘附性菌株的反应更迅速和实质性。这两种菌株诱导类似的变化模式（上调或下调）的肠，脾或肾细胞因子的表达，但他们在这些基因的反应程度不同。肠道HSP 70表达水平的变化与益生菌治疗之间的粘附性肠道细菌群落的相似性系数的变化一致。最高剂量的HALB似乎可以防止浸泡在A中的毒性作用。A组与B组比较差异有统计学意义（P < 0.05）。因此，乳酸杆菌菌株对肠道的粘附程度可能是筛选水产养殖益生菌菌株的有利标准。(c)2013爱思唯尔有限公司版权所有。
This study compares the effects of two Lactobacillus strains, highly adhesive Lactobacillus brevis JCM 1170 (HALB) and less-adhesive Lactobacillus acidophilus JCM 1132 (LALB), on the survival and growth, adhesive gut bacterial communities, immunity, and protection against pathogenic bacterial infection in juvenile hybrid tilapia. During a 5-week feeding trial the fish were fed a diet containing 0 to 10(9) cells/g feed of the two Lactobacillus strains. Samples of intestine, kidney, and spleen were taken at the start and at 10, 20, and 35 days for analysis of stress tolerance and cytokine gene mRNA levels and to assess the diversity of adhesive gut bacterial communities. A 14-day immersion challenge with Aeromonas hydrophila NJ-1 was also performed following the feeding trial. The results showed no significant differences in survival rate, weight gain, or feed conversion in the different dietary treatments. The adhesive gut bacterial communities were strikingly altered in the fish fed either the HALB or the LALB, but the response was more rapid and substantial with the adhesive strain. The two strains induced similar changes in the patterns (upregulation or downregulation) of intestinal, splenic or kidney cytokine expression, but they differed in the degree of response for these genes. Changes in intestinal HSP70 expression levels coincided with changes in the similarity coefficient of the adhesive gut bacterial communities between the probiotic treatments. The highest dose of the HALB appeared to protect against the toxic effects of immersion in A. hydrophila (P < 0.05). In conclusion, the degree to which Lactobacillus strains adhere to the gut may be a favorable criterion in selecting probiotic strain for aquaculture. (c) 2013 Elsevier Ltd. All rights reserved.