The in-planta induced ecp2 gene of the tomato pathogen Cladosporium fulvum is not essential for pathogenicity
The in-planta induced ecp2 gene of the tomato pathogen Cladosporium fulvum is not essential for pathogenicity
复制标题
番茄病原菌黄枝孢菌的植物内诱导的 ecp2 基因对于致病性并不是必需的
DOI：
10.1007/bf00309555
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发表时间：
1994
期刊：
影响因子：
2.5
通讯作者：
H. Broek
中科院分区：
文献类型：
--
作者：
R. Marmeisse;G. V. D. Ackerveken;T. Goosen;P. D. Wit;H. Broek
在番茄叶片定殖过程中，病原菌黄化枝孢菌在寄主组织细胞间隙分泌低分子蛋白质。这些蛋白质是由高表达的ecpgene编码的。虽然在植物中生长，但不表达，或只是微弱地表达。在体外生长的黄腐病。为了研究编码17-kDa蛋白ECP2的致病基因ecp2的功能，我们对该基因进行了两次连续的干扰。首先，ecp2基因被潮霉素B抗性基因盒打断。在第二次基因破坏中，ecp2基因从基因组中完全删除，取而代之的是一个金霉素抗性基因盒。两个中断突变体对番茄幼苗仍有致病作用，表明C。叶霉ecp2基因对番茄的致病性不是必需的。
During the colonization of tomato leaves, the fungal pathogenCladosporium fulvumexcretes low-molecular-weight proteins in the intercellular spaces of the host tissue. These proteins are encoded by theecpgenes which are highly expressed inC. fulvumwhile growingin plantabut are not, or are only weakly, expressed inC. fulvumgrown in vitro. To investigate the function of the putative pathogenicity geneecp2, encoding the 17-kDa protein ECP2, we performed two successive disruptions of the gene. In the first of these, theecp2 gene was interrupted by a hygromycin B resistance gene cassette. In the second gene disruption, theecp2 gene was completely deleted from the genome, and replaced by a phleomycin resistance gene cassette. Both disruption mutants were still pathogenic on tomato seedlings, indicating that theC. fulvum ecp2 gene is not essential for pathogenicity in tomato.