Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance
Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance
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番茄前系统蛋白基因在拟南芥中的表达揭示了其 mRNA 的系统易位并赋予坏死性真菌抗性
DOI：
10.1111/nph.14858
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发表时间：
2018
期刊：
影响因子：
9.4
通讯作者：
Lin Jinxing
中科院分区：
文献类型：
--
作者：
Zhang Haiyan;Yu Pengli;Zhao Jiuhai;Jiang Hongling;Li Chuanyou;Wang Haiyang;Zhu Yingfang;Botella Miguel A.;Samaj Jozef;Lin Jinxing
系统素（Systemin，缩写SYS）是一种由200个氨基酸前体（前系统素，PS）加工而成的十八肽激素，在番茄防御基因的系统激活中起着核心作用，以响应草食动物和病原体的攻击。我们在拟南芥中构建了带有绿色荧光蛋白（PS-GFP）标记的转基因番茄PS基因，该转基因番茄PS基因是利用茎或根特异性启动子和组成型35 S启动子构建的。用激光共聚焦显微镜观察PS-/E-GFP的亚细胞定位，用真实的荧光定量PCR检测基因转录本。拟南芥中PS-GFP的茎/根特异性表达和嫁接实验表明，PSmRNA以双向方式移动。我们还发现，PS的异位表达提高了植物对坏死营养型真菌灰葡萄孢的抗性，这与特定病原体响应基因的转录水平的显著上调一致。我们的研究结果为植物信号转导的多方面机制及其在基因工程中的潜在应用提供了新的见解，以提高不同植物家族的病原体抗性。
Systemin (SYS), an octadecapeptide hormone processed from a 200‐amino‐acid precursor (prosystemin, PS), plays a central role in the systemic activation of defense genes in tomato in response to herbivore and pathogen attacks. However, whetherPSmRNA is transferable and its role in systemic defense responses remain unknown.We created the transgenic tomatoPSgene tagged with the green fluorescent protein (PS‐GFP) using a shoot‐ or root‐specific promoter, and the constitutive35Spromoter inArabidopsis. Subcellular localization of PS‐/SYS‐GFP was observed using confocal laser scanning microscopy and gene transcripts were determined using quantitative real‐time PCR.InArabidopsis, PS protein can be processed and SYS is secreted. Shoot‐/root‐specific expression ofPS‐GFPinArabidopsis, and grafting experiments, revealed that thePSmRNA moves in a bi‐directional manner. We also found that ectopic expression ofPSimprovesArabidopsisresistance to the necrotrophic fungusBotrytis cinerea, consistent with substantial upregulation of the transcript levels of specific pathogen‐responsive genes.Our results provide novel insights into the multifaceted mechanism of SYS signaling transport and its potential application in genetic engineering for increasing pathogen resistance across diverse plant families.