Mps1 promotes chromosome meiotic chromosome biorientation through Dam1.
Mps1 promotes chromosome meiotic chromosome biorientation through Dam1.
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MPs1 通过 Dam1 促进染色体减数分裂染色体生物定向。
DOI：
10.1091/mbc.e17-08-0503
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发表时间：
2018
影响因子：
3.3
通讯作者：
Dawson,DeanS
中科院分区：
文献类型：
--
作者：
Meyer,RégisE;Brown,Jamin;Beck,Lindsay;Dawson,DeanS
在芽殖酵母减数分裂中，同源染色体通过交叉连接，然后在纺锤体上来回移动，直到它们双向取向，配偶体的动粒从相反的纺锤体两极附着到微管上。保守激酶Mps1中的某些突变导致灾难性的减数分裂分离错误，但有丝分裂缺陷轻微。我们测试是否Dam1，一个已知的底物Mps1，是必要的关键减数分裂的作用。我们发现，即使当Dam1不被Mps1磷酸化时，也会建立运动舞蹈微管连接，但Dam1的Mps1磷酸化维持了这些连接。但是，当Dam1没有磷酸化时，减数分裂缺陷并不像Mps1失活时那样灾难性。结果表明，Mps1的减数分裂的作用之一是稳定的连接，已建立动粒和微管之间的磷酸化Dam1。
In budding yeast meiosis, homologous chromosomes become linked by chiasmata and then move back and forth on the spindle until they are bioriented, with the kinetochores of the partners attached to microtubules from opposite spindle poles. Certain mutations in the conserved kinase, Mps1, result in catastrophic meiotic segregation errors but mild mitotic defects. We tested whether Dam1, a known substrate of Mps1, was necessary for its critical meiotic role. We found that kinetochore–microtubule attachments are established even when Dam1 is not phosphorylated by Mps1, but that Mps1 phosphorylation of Dam1 sustains those connections. But the meiotic defects when Dam1 is not phosphorylated are not nearly as catastrophic as when Mps1 is inactivated. The results demonstrate that one meiotic role of Mps1 is to stabilize connections that have been established between kinetochores and microtubles by phosphorylating Dam1.