Analysis of CD57+ natural killer cells and CD8+ T lymphocytes in periapical granulomas and radicular cysts
Analysis of CD57+ natural killer cells and CD8+ T lymphocytes in periapical granulomas and radicular cysts
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DOI：
10.1590/1807-3107bor-2017.vol31.0106
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发表时间：
2017-12-01
影响因子：
2.5
通讯作者：
Miguel, Márcia Cristina da Costa
中科院分区：
文献类型：
--
作者：
Silva, Luiz Arthur Barbosa da;Sá, Maria Alice Ramalho;Miguel, Márcia Cristina da Costa
本研究的目的是比较根尖肉芽肿（PG）和根尖囊肿（RC）中CD 57（+）自然杀伤（NK）细胞和CD 8（+）T淋巴细胞的数量。对25例PG和25例RC进行组织学分析和使用抗CD 57和抗CD 8生物标志物的免疫组化。在10个视野（放大400倍）中计数阳性细胞，并计算每种情况的中位数。根据病变类型、浸润强度和衬里上皮厚度，采用统计学检验评估CD 57（+）NK细胞和CD 8（+）T淋巴细胞数量的差异。PG中CD 57（+）NK细胞和CD 8（+）T淋巴细胞的数量高于RC（分别为p = 0.129和p = 0.541）。萎缩和增生上皮中CD 57（+）NK细胞数量的比较显示萎缩上皮中细胞数量较多（p = 0.042）。与I/II级浸润相比，在III级浸润中观察到更多的CD 57（+）NK细胞和CD 8（+）T淋巴细胞（分别为p = 0.145和p = 0.725）。当PG和RC单独或联合分析时，在大多数情况下，CD 8（+）T淋巴细胞比CD 57（+）NK细胞更普遍（p < 0.0001）。CD 57（+）NK细胞和CD 8（+）T淋巴细胞在抗病毒防御中起关键作用，这些细胞的存在支持了这些微生物参与PG和RC发病机制的证据。由CD 8（+）T淋巴细胞介导的应答更频繁，表明获得性免疫在这些慢性病变中的参与更大。
The aim of this study was to compare the number of CD57(+) natural killer (NK) cells and CD8(+) T lymphocytes between periapical granulomas (PGs) and radicular cysts (RCs). Twenty-fives cases of PGs and 25 of RCs were submitted to histological analysis and immunohistochemistry using anti-CD57 and anti-CD8 biomarkers. Positive cells were counted in 10 fields (400x magnification) and the median value was calculated for each case. Statistical tests were used to evaluate differences in the number of CD57(+) NK cells and CD8(+) T lymphocytes according to type of lesion, intensity of the infiltrate and thickness of the lining epithelium. The number of CD57(+) NK cells and CD8(+) T lymphocytes was higher in PGs than in RCs (p = 0.129 and p = 0.541, respectively). Comparison of the number of CD57(+) NK cells in atrophic and hyperplastic epithelium revealed a larger number of cells in the atrophic epithelium (p = 0.042). A larger number of CD57(+) NK cells and CD8(+) T lymphocytes were observed in grade III infiltrates compared to grade I/II (p = 0.145 and p = 0.725, respectively). CD8(+) T lymphocytes were more prevalent than CD57(+) NK cells in most cases when PGs and RCs were analyzed separately or in combination (p < 0.0001). CD57(+) NK cells and CD8(+) T lymphocytes play a key role in antiviral defense and the presence of these cells supports evidence suggesting the participation of these microorganisms in the pathogenesis of PGs and RCs. The response mediated by CD8(+) T lymphocytes was more frequent, indicating greater participation of the adaptive immunity in these chronic lesions.