On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation.
On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation.
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DOI：
10.1039/b815765k
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发表时间：
2009-05
期刊：
影响因子：
6.1
通讯作者：
H. Sugino;K. Ozaki;Y. Shirasaki;T. Arakawa;S. Shoji;T. Funatsu
中科院分区：
文献类型：
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作者：
H. Sugino;K. Ozaki;Y. Shirasaki;T. Arakawa;S. Shoji;T. Funatsu
微流控平台是诊断和生物医学研究的重要工具，因为它使我们能够安全地处理珍贵的细胞和感染性材料。我们开发了一种具有荧光光谱检测和多路分离功能的片上微流控分选器。使用 1 x 16 阵列光电倍增管每 2 ms 获取一次微通道中样本的荧光光谱 (495-685 nm)。根据我们之前报道的热可逆凝胶聚合物技术，通过光谱识别样本并收集到相应的通道中（Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita and T. Funatsu, Anal. Chem., 2006, 78, 695-701）。成功分离了表达不同荧光蛋白的四种荧光微球和三种大肠杆菌细胞，分离精度和纯度均优于 90%，吞吐量约为每秒 1 个颗粒。
The microfluidic platform is an important tool for diagnosis and biomedical studies because it enables us to handle precious cells and infectious materials safely. We have developed an on-chip microfluidic sorter with fluorescence spectrum detection and multiway separation. The fluorescence spectrum of specimens (495-685 nm) in the microchannels was obtained every 2 ms using a 1 x 16 arrayed photomultiplier tube. The specimen was identified by its spectrum and collected into the corresponding channel based on our previously reported thermoreversible gelation polymer technique (Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita and T. Funatsu, Anal. Chem., 2006, 78, 695-701). Four kinds of fluorescence microspheres and three kinds of Escherichia coli cells, expressing different fluorescent proteins, were successfully separated with accuracy and purity better than 90% at a throughput of about one particle per second.