Land Management and Microbial Seed Load Effect on Rhizosphere and Endosphere Bacterial Community Assembly in Wheat
Land Management and Microbial Seed Load Effect on Rhizosphere and Endosphere Bacterial Community Assembly in Wheat
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DOI：
10.3389/fmicb.2019.02625
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发表时间：
2019-11-15
影响因子：
5.2
通讯作者：
Mauchline, Tim H.
中科院分区：
文献类型：
--
作者：
Kavamura, Vanessa Nessner;Robinson, Rebekah J.;Mauchline, Tim H.
微生物群落生态学研究传统上利用基于培养的方法，尽管下一代扩增子测序的出现促进了复杂微生物群落的上级分辨率分析。在这里，我们使用文化依赖和独立的方法来探讨土地利用以及微生物种子负载对小麦根际和根际细菌群落结构的影响。结果发现，生态位是一个重要的因素，在塑造微生物组时，使用这两种方法的方法，土地利用也是一个歧视性因素的文化独立的方法。虽然文化独立的方法提供了更高的分辨率的分析，它被发现，在根际，特定的操作分类单位（OTU）的文化依赖的部分是不存在的文化独立的部分，这表明，需要更深入的序列分析，这种方法是详尽的。我们还发现，微生物种子负载定义的土壤中生长的植物，这是不小麦适应的内圈，而不是根际，社区结构。总之，这些发现增加了我们对土地管理和微生物种子负荷在塑造小麦根部微生物组方面的重要性的了解，这些知识将促进利用植物-微生物相互作用来开发新型微生物接种剂。
Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant-microbe interactions for the development of novel microbial inoculants.