Camera-based three-dimensional real-time particle tracking at kHz rates and Ångström accuracy.
Camera-based three-dimensional real-time particle tracking at kHz rates and Ångström accuracy.
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DOI：
10.1038/ncomms6885
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发表时间：
2015-01-07
影响因子：
16.6
通讯作者：
Seidel, Ralf
中科院分区：
文献类型：
--
作者：
Huhle, Alexander;Klaue, Daniel;Brutzer, Hergen;Daldrop, Peter;Joo, Sihwa;Otto, Oliver;Keyser, Ulrich F.;Seidel, Ralf
光镊和磁镊被广泛用于探测单个生物分子复合物的力学和活性。他们依靠微米大小的颗粒来检测来自颗粒位置的分子构象变化。到目前为止，只有通过光电二极管使用激光检测才能实现具有100 μ m精度的实时颗粒跟踪。在这里，我们证明了基于相机的成像可以为所有三个维度提供类似的性能。以kHz速率的粒子成像与由图形处理单元加速的实时数据处理相结合。对于固定在样品池中的颗粒，我们可以检测到由以10 Hz速率的细胞平移引入的3 μ m大小的步长，而对于DNA束缚的颗粒，可以分辨出以1 Hz的5 μ m步长。此外，可以并行跟踪20个粒子，具有相当的精度。我们的方法为一次使用多个粒子的高分辨率镊子实验提供了一种简单而强大的方法。
Optical and magnetic tweezers are widely employed to probe the mechanics and activity of individual biomolecular complexes. They rely on micrometer-sized particles to detect molecular conformational changes from the particle position. Real-time particle tracking with Ångström accuracy has so far been only achieved using laser detection through photodiodes. Here we demonstrate that camera-based imaging can provide a similar performance for all three dimensions. Particle imaging at kHz rates is combined with real-time data processing being accelerated by a graphics processing unit. For particles that are fixed in the sample cell we can detect 3 Å sized steps that are introduced by cell translations at rates of 10 Hz, while for DNA-tethered particles 5 Å steps at 1 Hz can be resolved. Moreover, 20 particles can be tracked in parallel with comparable accuracy. Our approach provides a simple and robust way for high-resolution tweezers experiments using multiple particles at a time.
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影响因子：
16.6
作者：
Klaue D;Kobbe D;Kemmerich F;Kozikowska A;Puchta H;Seidel R
通讯作者：
Seidel R
影响因子：
7.7
作者：
Qi Z;Pugh RA;Spies M;Chemla YR
通讯作者：
Chemla YR
DOI：
10.1126/science.1225437
发表时间：
2012-11-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Manosas M;Perumal SK;Croquette V;Benkovic SJ
通讯作者：
Benkovic SJ
影响因子：
19.6
作者：
Keyser, Ulrich F.;Koeleman, Bernard N.;Dekker, Cees
通讯作者：
Dekker, Cees
影响因子：
10.8
作者：
De Vlaminck, Iwijn;Henighan, Thomas;Dekker, Cees
通讯作者：
Dekker, Cees