Structure of adenosine deaminase mRNAs from normal and adenosine deaminase-deficient human cell lines.
Structure of adenosine deaminase mRNAs from normal and adenosine deaminase-deficient human cell lines.
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来自正常和腺苷脱氨酶缺陷的人类细胞系的腺苷脱氨酶 mRNA 的结构。
DOI：
10.1128/mcb.4.9.1712-1717.1984
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发表时间：
1984
影响因子：
5.3
通讯作者：
Hutton,JJ
中科院分区：
文献类型：
--
作者：
Adrian,GS;Wiginton,DA;Hutton,JJ
对正常和突变淋巴母细胞的人腺苷脱氨酶mRNA进行了序列分析，并对mRNA-cDNA杂种S1酶切产生的DNA片段进行了电泳分析。克隆cDNA的1,533碱基序列代表了完整的mRNA序列，可能除了一些5 '未翻译区。对克隆cDNA和正常腺苷脱氨酶mRNA杂交的S1核酸酶分析证实，先前检测的腺苷脱氨酶cDNA中的76个碱基序列是一个内含子。对7个突变细胞系的mrna进行S1核酸酶分析表明，GM-2471、GM-2756、GM-4258和GM-2606细胞中的4个突变体含有小缺陷，如单碱基变化，这些缺陷是S1核酸酶技术无法检测到的。GM-3043、GM-2294和GM-2825A细胞中的三种mrna确实含有S1核酸酶可检测到的缺陷。这些缺陷彼此不同，并被映射到mRNA的特定区域。一些或所有这些有缺陷的mrna被认为是由异常RNA加工引起的。
The structure of human adenosine deaminase mRNA from normal and mutant lymphoblasts was examined by sequence analysis of a cDNA for normal mRNA and electrophoretic analyses of DNA fragments generated by S1 endonuclease cleavage of mRNA-cDNA hybrids. The 1,533-base sequence of the cloned cDNA represents the complete mRNA sequence with the possible exception of some of the 5′ untranslated region. S1 nuclease analyses of hybrids between cloned cDNA and normal adenosine deaminase mRNA confirmed that a 76-base sequence in a previously examined adenosine deaminase cDNA is an intron. S1 nuclease analyses of mRNAs from seven mutant cell lines demonstrated that four of the mutants, those in the GM-2471, GM-2756, GM-4258, and GM-2606 cells, contain small defects, such as single-base changes, that are not detectable by the S1 nuclease technique. Three of the mRNAs, those in GM-3043, GM-2294, and GM-2825A cells, do contain defects detectable with S1 nuclease. These defects differ from each other and have been mapped to specific regions of the mRNA. Some or all of these defective mRNAs are postulated to result from anomalous RNA processing.